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猪传染性胃肠炎病毒刺突蛋白在重组植物乳杆菌中诱导的免疫应答。

Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets.

机构信息

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.

出版信息

Appl Microbiol Biotechnol. 2018 Oct;102(19):8403-8417. doi: 10.1007/s00253-018-9205-0. Epub 2018 Jul 18.

DOI:10.1007/s00253-018-9205-0
PMID:30022263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7080080/
Abstract

Transmissible gastroenteritis coronavirus (TGEV) is one of the most severe threats to the swine industry. In this study, we constructed a suite of recombinant Lactobacillus plantarum with surface displaying the spike (S) protein coming from TGEV and fused with DC cells targeting peptides (DCpep) to develop an effective, safe, and convenient vaccine against transmissible gastroenteritis. Our research results found that the recombinant Lactobacillus plantarum (NC8-pSIP409-pgsA-S-DCpep) group expressing S fused with DCpep could not only significantly increase the percentages of MHC-IICD80 B cells and CD3CD4 T cells but also the number of IgA B cells and CD3CD4 T cells of ileum lamina propria, which elevated the specific secretory immunoglobulin A (SIgA) titers in feces and IgG titers in serum. Taken together, these results suggest that NC8-pSIP409-pgsA-S-DCpep expressing the S of TGEV fused with DCpep could effectively induce immune responses and provide a feasible original strategy and approach for the design of TGEV vaccines.

摘要

传染性胃肠炎冠状病毒（TGEV）是猪产业面临的最严重威胁之一。在本研究中，我们构建了一系列表面展示来自 TGEV 的刺突（S）蛋白并融合了树突状细胞靶向肽（DCpep）的重组植物乳杆菌，以开发针对传染性胃肠炎的有效、安全且方便的疫苗。我们的研究结果发现，表达融合了 DCpep 的 S 的重组植物乳杆菌（NC8-pSIP409-pgsA-S-DCpep）组不仅能显著增加 MHC-IICD80 B 细胞和 CD3CD4 T 细胞的百分比，还能增加回肠固有层中 IgA B 细胞和 CD3CD4 T 细胞的数量，从而提高粪便中的特异性分泌型免疫球蛋白 A（SIgA）滴度和血清中的 IgG 滴度。综上所述，这些结果表明，表达融合了 DCpep 的 TGEV S 的 NC8-pSIP409-pgsA-S-DCpep 能够有效诱导免疫应答，为 TGEV 疫苗的设计提供了一种可行的原始策略和方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/bcea01242574/253_2018_9205_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/c57dbc0e3ea0/253_2018_9205_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/58e8aa9e9215/253_2018_9205_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/a02862b276ed/253_2018_9205_Fig3a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/0eed4196ebb8/253_2018_9205_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/3b69cd5aad47/253_2018_9205_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/ebaca604b3fc/253_2018_9205_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/2ea7626b3c8f/253_2018_9205_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/d390cca4ac98/253_2018_9205_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/bcea01242574/253_2018_9205_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/c57dbc0e3ea0/253_2018_9205_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/58e8aa9e9215/253_2018_9205_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/a02862b276ed/253_2018_9205_Fig3a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/0eed4196ebb8/253_2018_9205_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/3b69cd5aad47/253_2018_9205_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/ebaca604b3fc/253_2018_9205_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/2ea7626b3c8f/253_2018_9205_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/d390cca4ac98/253_2018_9205_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1294/7080080/bcea01242574/253_2018_9205_Fig9_HTML.jpg

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