Adams W T, Nolan Michael W, Ivanisevic Albena
Department of Materials Science and Engineering, North Carolina State University, 911 Partners Way, Raleigh, North Carolina 27695, United States.
Department of Clinical Sciences (College of Veterinary Medicine), and Comparative Medicine Institute, North Carolina State University, 1060 William Moore Drive, Raleigh, North Carolina 27606, United States.
ACS Omega. 2018 May 31;3(5):5252-5259. doi: 10.1021/acsomega.8b00524. Epub 2018 May 15.
The reported results test the effects of the collective behavior hypothesized to contribute to the production of more reactive oxygen species (ROS) in vitro and result in an enhanced radiosensitization. The role of particle shape in composites with gallium oxyhydroxide (GaOOH) particles and Matrigel is studied. Particles of two different shapes are embedded into the gel to understand only the materials effect on the generation of ROS rather than cell penetrating variations. The paper reports materials characterization by scanning electron microscopy and X-ray diffraction. The stability of the particles within the composite is assessed by quantification of leached metal using inductively coupled plasma mass spectrometry. The amount of ROS in each construct under variable radiation conditions is quantified in the presence and absence of PC12 cells seeded on top of the composites. The viability of cells is also recorded under different in vitro conditions. The collective materials characterization and the results from the bioassays are used to explain the role of anisotropy on the radiosensitization of nanostructures containing Ga. The presence of Ga ions in composites can have a radiosensitizing effect, and the amount of the available Ga determines the magnitude of the radiosensitization. The shape of the particles determines the stability in aqueous solutions and release of Ga that triggers ROS production. The concentration and shape of Ga-containing materials can be combined to generate an additive effect by increasing the amount of available free metal ions in solution. The studies with GaOOH containing composites enable one to explore the role of key parameters that lead to an increased efficiency of radiation treatments.
所报道的结果测试了假设的集体行为在体外产生更多活性氧（ROS）并导致放射增敏作用增强方面的影响。研究了粒子形状在含氢氧化镓（GaOOH）粒子与基质胶的复合材料中的作用。将两种不同形状的粒子嵌入凝胶中，以仅了解材料对ROS生成的影响，而非细胞穿透差异的影响。该论文报道了通过扫描电子显微镜和X射线衍射进行的材料表征。使用电感耦合等离子体质谱法通过定量浸出金属来评估复合材料中粒子的稳定性。在复合材料顶部接种或未接种PC12细胞的情况下，对不同辐射条件下每个构建体中的ROS量进行定量。还记录了不同体外条件下细胞的活力。集体材料表征和生物测定结果用于解释各向异性对含镓纳米结构放射增敏作用的作用。复合材料中Ga离子的存在可产生放射增敏作用，可用Ga的量决定了放射增敏的程度。粒子形状决定了在水溶液中的稳定性以及触发ROS产生的Ga的释放。含Ga材料的浓度和形状可通过增加溶液中可用游离金属离子的量来产生累加效应。对含GaOOH复合材料的研究使人们能够探索导致放射治疗效率提高的关键参数的作用。
