Goswami Upashi, Sahoo Amaresh Kumar, Chattopadhyay Arun, Ghosh Siddhartha Sankar
Centre for Nanotechnology, Department of Chemistry, and Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Department of Applied Science, Indian Institute of Information Technology, Allahabad, Allahabad, Uttar Pradesh 211012, India.
ACS Omega. 2018 Jun 30;3(6):6113-6119. doi: 10.1021/acsomega.8b00504. Epub 2018 Jun 6.
Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL for the Gram-positive bacteria. Brief treatment with lysozyme could differentiate the Gram strains based on their luminescence intensities. The current method could also detect bacterial contaminants from water sources and kanamycin-resistant strains rapidly. This quick synthesis of Au NCs on a bacterial template attributes an easy and rapid method for enumeration and detection of bacterial contaminants and kanamycin-resistant strains.
在此，我们介绍一种在细菌表面合成发光金纳米簇（Au NC）的简便新方法，用于检测、计数和菌株鉴别。革兰氏阴性菌的检测限为740±14菌落形成单位（CFU）/mL，革兰氏阳性菌的检测限为634±16 CFU/mL。用溶菌酶进行短暂处理可根据革兰氏菌株的发光强度进行区分。该方法还能快速检测水源中的细菌污染物和卡那霉素抗性菌株。在细菌模板上快速合成金纳米簇为细菌污染物和卡那霉素抗性菌株的计数和检测提供了一种简便快捷的方法。
