Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Institut Cochin U1016 Equipe Batteux, Laboratoire de Dermatologie-CNR Syphilis, Faculté de Médecine, Université Sorbonne Paris Descartes, Paris, France.
PLoS One. 2018 Jul 19;13(7):e0201068. doi: 10.1371/journal.pone.0201068. eCollection 2018.
Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using the recently developed Multilocus Sequence Typing system. The samples (n = 133) used in this study were collected from 2010-2016 from patients with diagnosed primary or secondary syphilis attending outpatient centers or hospitals in several locations in France. Altogether, 18 different allelic profiles were found among the fully typed samples (n = 112). There were five allelic variants identified for TP0136, 12 for TP0548, and eight for TP0705. Out of the identified alleles, one, seven, and three novel alleles were identified in TP0136, TP0548, and TP0705, respectively. Partial allelic profiles were obtained from 6 samples. The majority of samples (n = 110) belonged to the SS14-like cluster of TPA isolates while 7 clustered with Nichols-like isolates. Patients infected with Nichols-like samples were more often older (p = 0.041) and more often diagnosed with secondary syphilis (p = 0.033) compared to patients infected with SS14-like samples. In addition, macrolide resistance caused by the A2058G mutation was found to be associated with allelic profile 1.3.1 or with strains belonging to the 1.3.1 lineage (p<0.001). The genetic diversity among TPA strains infecting the European population was surprisingly high, which suggests that additional studies are needed to reveal the full genetic diversity of TPA pathogens infecting humans.
苍白密螺旋体亚种苍白密螺旋体，性传播梅毒的病原体，在法国临床样本中检测到，使用最近开发的多位点序列分型系统进行分子分型。本研究使用的样本（n=133）于 2010-2016 年从法国多个地点的门诊中心或医院就诊的诊断为原发性或二期梅毒的患者中收集。在完全分型的样本（n=112）中，共发现 18 种不同的等位基因谱。TP0136 有 5 种等位基因变异，TP0548 有 12 种，TP0705 有 8 种。在所鉴定的等位基因中，在 TP0136、TP0548 和 TP0705 中分别鉴定出一个、七个和三个新的等位基因。从 6 个样本中获得部分等位基因谱。大多数样本（n=110）属于 TPA 分离株的 SS14 样簇，而 7 个样本与 Nichols 样分离株聚类。与感染 SS14 样样本的患者相比，感染 Nichols 样样本的患者年龄更大（p=0.041），且更常诊断为二期梅毒（p=0.033）。此外，A2058G 突变引起的大环内酯类耐药与等位基因谱 1.3.1 或属于 1.3.1 谱系的菌株有关（p<0.001）。感染欧洲人群的 TPA 菌株的遗传多样性令人惊讶地高，这表明需要进一步研究以揭示感染人类的 TPA 病原体的全部遗传多样性。
