Niu Lili, Xiong Mengjie, Zhang Juan, Xiang Yangquan, Song Lei, Hua Ziyi, Li Wenying
Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2018 Feb;68(2):564-569. doi: 10.1099/ijsem.0.002542. Epub 2018 Jan 4.
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1, was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1 was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435 (98.4 %), Bacillus acidicolaDSM 14745 (97.6 %), Bacillus paralicheniformis KACC 18426 (97.2 %) and Bacillus ginsengihumi KCTC 13944 (96.7 %). The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1 to the genus Bacillus. The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-7 (95 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid. The average nucleotide identity values between strain 7578-1 and its most closely related species were 67.8-82.4 % by OrthoANIu analysis. The DNA-DNA relatedness value between strain 7578-1 and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1 represented a novel species in the genus Bacillus. The DNA G+C content of strain 7578-1 was 36.0 mol%. On the basis of the presented polyphasic evidence, strain 7578-1 is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp. nov. The type strain is 7578-1 (=CGMCC 1.15374=KCTC 33845).
从成熟的普洱茶中分离出一株新型需氧、革兰氏染色阳性、产芽孢的杆状细菌菌株7578-1。基于16S rRNA基因序列相似性比较，菌株7578-1被归入芽孢杆菌属，并且似乎与模式菌株沙克尔顿芽孢杆菌LMG 18435（相似度98.4%）、嗜酸芽孢杆菌DSM 14745（相似度97.6%）、类地衣芽孢杆菌KACC 18426（相似度97.2%）和人参土芽孢杆菌KCTC 13944（相似度96.7%）密切相关。含有主要脂肪酸异C15:0、anteiso-C15:0和anteiso-C17:0的脂肪酸谱支持将菌株7578-1归入芽孢杆菌属。该菌株具有A1γ型细胞壁肽聚糖，以中-二氨基庚二酸作为诊断性二氨基酸。主要甲基萘醌为MK-7（95%）。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、一种未鉴定的磷脂和一种未鉴定的脂质。通过OrthoANIu分析，菌株7578-1与其最密切相关物种之间的平均核苷酸同一性值为67.8-82.4%。菌株7578-1与密切相关物种的模式菌株之间的DNA-DNA相关性值为17-39%，这再次表明菌株7578-1代表芽孢杆菌属中的一个新物种。菌株7578-1的DNA G+C含量为36.0 mol%。基于所提供的多相证据，菌株7578-1被认为代表芽孢杆菌属的一个新物种，我们将其命名为茶芽孢杆菌（Bacillus camelliae sp. nov.）。模式菌株为7578-1（=CGMCC 1.15374=KCTC 33845）。
