Rapoport S I, Lust W D, Fredericks W R
Exp Neurol. 1986 Feb;91(2):319-30. doi: 10.1016/0014-4886(86)90072-5.
An in vivo brain perfusion technique was used to examine effects of hypoxia on cerebral cortical metabolism in barbiturate-anesthetized rats. Dulbecco's phosphate-buffered solution (PBS), or Dulbecco's PBS + 6 mM glucose, was infused into the right carotid circulation for 0 to 3 min, at a rate that reduced regional cerebral blood flow to the ipsilateral parietal lobe by more than 40% and O2 delivery by about 50%. The duration of infusion of either solution was correlated negatively with the ipsilateral parietal lobe concentrations of glucose, ATP, and phosphocreatine (PCr), and positively with parietal concentrations of lactate and cAMP. cGMP increased in relation to infusion duration of Dulbecco's PBS. Statistically significant elevations of brain lactate occurred after 1 min of infusion of Dulbecco's PBS; lactate was elevated and glucose was reduced after 2 min of infusion of either solution. Brain ATP, PCr, and glycogen concentrations decreased in relation to the elevation in brain lactate, and the [PCr]:[ATP] ratio declined. The results demonstrated that limited hypoxia stimulated cerebral glycolysis and produced a concurrent decrease in brain ATP and PCr. However, ATP was spared to a degree, at the expense of PCr.
采用一种体内脑灌注技术，研究了缺氧对巴比妥麻醉大鼠大脑皮质代谢的影响。将杜尔贝科磷酸盐缓冲液（PBS）或杜尔贝科PBS + 6 mM葡萄糖注入右侧颈动脉循环0至3分钟，灌注速率使同侧顶叶的局部脑血流量减少超过40%，氧输送量减少约50%。两种溶液的灌注持续时间与同侧顶叶葡萄糖、ATP和磷酸肌酸（PCr）的浓度呈负相关，与顶叶乳酸和cAMP的浓度呈正相关。cGMP随着杜尔贝科PBS的灌注持续时间而增加。灌注杜尔贝科PBS 1分钟后，脑乳酸出现统计学上的显著升高；灌注两种溶液2分钟后，乳酸升高而葡萄糖降低。脑ATP、PCr和糖原浓度随着脑乳酸的升高而降低，且[PCr]:[ATP]比值下降。结果表明，有限的缺氧刺激了脑糖酵解，并同时导致脑ATP和PCr减少。然而，ATP在一定程度上得以保留，代价是PCr。
