Department of Chemical Engineering, Institute of Technology, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Department of Chemical and Environmental Engineering, University of Basque Country, San Sebastian, Spain.
Sci Total Environ. 2018 Dec 15;645:533-542. doi: 10.1016/j.scitotenv.2018.07.155. Epub 2018 Jul 18.
A large amount of olive-derived biomass is generated yearly in Spain, which could be used as a potential source of bioactive compounds. The present work evaluates the recovery of natural antioxidants from olive tree pruning (OTP) and olive mill leaves (OML). For this purpose, the effect of different solvents on the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity was evaluated. The solvent was found to have a significant effect (p < 0.05) on the TPC, TFC, and the DPPH, ABTS, and FRAP activity, affording similar results for the extracts from the two by-products. The extracts obtained using 50% ethanol showed high TPC (23.85 and 27.54 mg GAE/g for OTP and OML, respectively) and TFC (52.82 and 52.39 mg RE/g for OTP and OML, respectively). Also, the OTP and OML extracts exhibited notable antioxidant activity as measured by the ABTS method (45.96 and 42.71 mg TE/g, respectively). Using pyrolysis-gas chromatography/mass spectrometry, 30 bioactive compounds were detected in both extracts. Additionally, UPLC-DAD-ESI-MS allowed the identification of 15 compounds in the samples. Furthermore, the antioxidant extracts were found to inhibit the growth of several food pathogenic bacteria. This research demonstrates that these by-products from olive grove farming are a good source of antioxidant compounds with antibacterial properties, which have potential applications in the food and pharmaceutical industries.
在西班牙，每年都会产生大量的橄榄衍生生物量，可以将其用作生物活性化合物的潜在来源。本工作评估了从橄榄树修剪（OTP）和橄榄磨叶（OML）中回收天然抗氧化剂的方法。为此，评估了不同溶剂对总酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性的影响。结果表明，溶剂对 TPC、TFC 和 DPPH、ABTS 和 FRAP 活性均有显著影响（p<0.05），两种副产物的提取物结果相似。使用 50%乙醇获得的提取物具有较高的 TPC（OTP 和 OML 分别为 23.85 和 27.54 mg GAE/g）和 TFC（OTP 和 OML 分别为 52.82 和 52.39 mg RE/g）。此外，OTP 和 OML 提取物在 ABTS 法测定的抗氧化活性方面表现出显著的活性（分别为 45.96 和 42.71 mg TE/g）。通过热解气相色谱/质谱联用仪，在两种提取物中检测到 30 种生物活性化合物。此外，UPLC-DAD-ESI-MS 允许鉴定样品中的 15 种化合物。此外，抗氧化提取物被发现能够抑制几种食源性病原体的生长。这项研究表明，橄榄种植园的这些副产物是具有抗菌特性的抗氧化化合物的良好来源，它们在食品和制药行业具有潜在的应用价值。
