Cooper R, Papaconstantinou J
J Biol Chem. 1986 Feb 5;261(4):1849-53.
Three alpha 1-acid glycoprotein (AGP) cDNA clones have been isolated from a mouse liver library. Restriction enzyme mapping and sequencing of these cDNAs have shown that two, pMAGP2 and pMAGP3, are virtually identical, whereas the third, pMAGP4, differs significantly both in sequence and restriction enzyme sites. The sequences are 91% identical and differ from each other by single base differences exclusively. No frameshifts are observed, and no termination codons are generated by the single base differences. We interpret these data to indicate that there are at least two distinct AGP genes in the mouse and that two species of AGP mRNA are formed by the transcription of these genes in the liver. Based on the large number of single amino acid substitutions previously observed in human AGP (Schmid, K., Kaufmann, H., Isemura, S., Bauer, F., Emura, J., Motoyama, T., Ishiguro, M., and Nanno, S. (1973) Biochemistry 12, 2711-2724), we propose that at least two functional AGP genes may also exist in humans.
已从小鼠肝脏文库中分离出三个α1-酸性糖蛋白（AGP）cDNA克隆。对这些cDNA进行限制性酶切图谱分析和测序表明，其中两个，即pMAGP2和pMAGP3，几乎完全相同，而第三个，pMAGP4，在序列和限制性酶切位点上都有显著差异。这些序列的同源性为91%，彼此之间仅存在单个碱基差异。未观察到移码现象，单个碱基差异也未产生终止密码子。我们认为这些数据表明小鼠中至少存在两个不同的AGP基因，并且这两种AGP mRNA是由这些基因在肝脏中的转录形成的。基于先前在人AGP中观察到的大量单氨基酸替换现象（施密德，K.，考夫曼，H.，石村，S.，鲍尔，F.，江村，J.，本山，T.，石黑，M.，和南野，S.（1973年）《生物化学》12卷，2711 - 2724页），我们推测人类中可能也至少存在两个功能性AGP基因。
