Fan C M, Maniatis T
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
EMBO J. 1989 Jan;8(1):101-10. doi: 10.1002/j.1460-2075.1989.tb03353.x.
We show that the human beta-interferon gene promoter contains two different virus-inducible regulatory elements, PRDI and PRDII (positive regulatory domains I and II). A single copy of either element alone has no discernible transcriptional activity in mouse fibroblasts. However, multiple copies of either element function as a constitutive or virus-inducible transcription element depending on the cell line in which the sequence was tested. These results in conjunction with previous studies suggest that virus induction of the human beta-interferon gene is achieved through cooperative interactions between two entirely distinct virus-inducible elements. Comparison of the properties of these elements reveals that multiple copies of PRDI, but not PRDII, can be activated by three different inducers, beta-IFN, gamma-IFN and virus. These results suggest that the pathways of virus and interferon induction may share at least one common regulatory component.
我们发现人类β-干扰素基因启动子包含两个不同的病毒诱导调控元件，即PRDI和PRDII（正调控区I和II）。单独的任一元件的单拷贝在小鼠成纤维细胞中均无明显的转录活性。然而，任一元件的多个拷贝根据测试序列所在的细胞系，可作为组成型或病毒诱导型转录元件发挥作用。这些结果与先前的研究共同表明，人类β-干扰素基因的病毒诱导是通过两个完全不同的病毒诱导元件之间的协同相互作用实现的。对这些元件特性的比较表明，PRDI的多个拷贝而非PRDII的多个拷贝可被三种不同的诱导剂β-干扰素、γ-干扰素和病毒激活。这些结果表明，病毒诱导途径和干扰素诱导途径可能至少共享一个共同的调控成分。
