Dente L, Ciliberto G, Cortese R
Nucleic Acids Res. 1985 Jun 11;13(11):3941-52. doi: 10.1093/nar/13.11.3941.
We have determined the sequence coding for human alpha 1-acid glycoprotein from two independently isolated cDNA clones and a genomic clone. The aminoacid sequences deduced from the three clones, deriving from three different individuals, are identical. Southern blot analysis on human DNA indicates that there are at least two genes coding for alpha 1-AGP. We propose that alpha 1-AGP found in plasma is a mixture of the products of these two different genes. This is the simpler explanation for the heterogeneity in the aminoacid composition in purified alpha 1-AGP observed by Schmid et al. (1). DNA sequence comparison with cDNA clones coding for human alpha 1-antitrypsin and haptoglobin shows a conserved sequence within the 5' untranslated region which may play a role in the acute phase response.
我们从两个独立分离的cDNA克隆和一个基因组克隆中确定了编码人α1-酸性糖蛋白的序列。从这三个克隆推导出来的氨基酸序列相同，这三个克隆来自三个不同的个体。对人类DNA的Southern印迹分析表明，至少有两个基因编码α1-AGP。我们提出，血浆中发现的α1-AGP是这两个不同基因产物的混合物。这是对Schmid等人（1）观察到的纯化α1-AGP氨基酸组成异质性的更简单解释。与编码人α1-抗胰蛋白酶和触珠蛋白的cDNA克隆进行DNA序列比较，发现在5'非翻译区内有一个保守序列，该序列可能在急性期反应中起作用。
