Chen Xindi, Shi Yaqin, Wang Tengyu, Liu Chunxia, Wang Wenlong, Wang Yu
Key Laboratory of Animal Disease Clinical Diagnosis and Treatment Technology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Key Laboratory of Animal Disease Clinical Diagnosis and Treatment Technology, College of Life Science, Inner Mongolia Agricultural University, Hohhot, China.
Front Vet Sci. 2025 May 12;12:1567869. doi: 10.3389/fvets.2025.1567869. eCollection 2025.
() is a common gastrointestinal nematode in small ruminants, posing a significant threat to the livestock industry. The control of often depends on drugs such as albendazole. However, the prolonged and improper use of these drugs by livestock producers has led to widespread resistance among ruminant populations, posing a major challenge to parasite management. It is reported that the resistance of to albendazole is related to the single nucleotide polymorphism (SNP) of β-tubulin homologous type I gene, but whether other genes are involved has not been reported.
Based on the comparative analysis of the transcriptome sequencing data of albendazole-sensitive and albendazole-resistant strains of , HCON_00043720 (Receptor-type guanylate cyclase GCY-12, GCY-12) gene was selected as the research object from the 23 differential genes in the cyclic guanosine monophosphate (cGMP) signaling pathway where the growth and development of dauer stage is located. The GCY-12 gene of eggs was silenced by RNA interference (RNAi) test. The reaction temperature was optimized, the expression of silenced eggs was detected by quantitative real-time polymerase chain reaction (qRT-PCR) technology, and the drug resistance of silenced eggs was detected by egg hatch assay (EHA).
The results showed that the gene expression level decreased significantly after GCY-12 interference, and the sensitivity of to albendazole increased.
This study highlights the potential role of GCY-12 in modulating albendazole resistance in H. contortus, offering new insights for developing effective therapeutic approaches.
（某线虫名称）是小型反刍动物常见的胃肠道线虫，对畜牧业构成重大威胁。对其的防控通常依赖于阿苯达唑等药物。然而，养殖户长期且不当使用这些药物导致反刍动物群体中广泛产生耐药性，给寄生虫管理带来重大挑战。据报道，（某线虫名称）对阿苯达唑的耐药性与β - 微管蛋白同源I型基因的单核苷酸多态性（SNP）有关，但是否涉及其他基因尚未见报道。
基于对（某线虫名称）阿苯达唑敏感株和耐药株转录组测序数据的比较分析，从（某线虫名称） dauer期生长发育所在的环磷酸鸟苷（cGMP）信号通路中的23个差异基因中选择HCON_00043720（受体型鸟苷酸环化酶GCY - 12，GCY - 12）基因作为研究对象。通过RNA干扰（RNAi）试验使（某线虫名称）虫卵的GCY - 12基因沉默。优化反应温度，采用定量实时聚合酶链反应（qRT - PCR）技术检测沉默虫卵的表达情况，并通过虫卵孵化试验（EHA）检测沉默虫卵的耐药性。
结果表明，GCY - 12干扰后基因表达水平显著下降，（某线虫名称）对阿苯达唑的敏感性增加。
本研究突出了GCY - 12在调节捻转血矛线虫对阿苯达唑耐药性中的潜在作用，为开发有效的治疗方法提供了新的见解。
