Richman D D, Buckmaster A, Bell S, Hodgman C, Minson A C
J Virol. 1986 Feb;57(2):647-55. doi: 10.1128/JVI.57.2.647-655.1986.
A type-specific monoclonal antibody, LP10, precipitated a glycoprotein with a molecular weight of approximately 59,000 from purified herpes simplex virus type 1. Although this glycoprotein was similar in size to glycoprotein D (gD), it was shown to be less abundant in both virions and infected cells, to migrate more rapidly in its precursor form, to incorporate glucosamine but not mannose, and to have a more stable precursor in tunicamycin-treated cells than the gD precursor (pgD). Immunoassays of cells infected with insertion recombinants and intertypic recombinants localized the gene coding for the target antigen of LP10 to the unique short (Us) region at map units 0.892 to 0.924 excluding gD. The target antigen of LP10 was then definitively mapped to the Us4 open reading frame by immunoprecipitation of a polypeptide synthesized by in vitro translation of a Us4-specific transcript prepared by using an SP6 cloning This newly identified glycoprotein product of the Us4 gene of herpes simplex virus type 1 is distinct from the previously identified gB1, gC1, gE1, and gH1.
一种型特异性单克隆抗体LP10从纯化的1型单纯疱疹病毒中沉淀出一种分子量约为59,000的糖蛋白。尽管这种糖蛋白的大小与糖蛋白D（gD）相似，但研究表明它在病毒粒子和受感染细胞中的含量均较少，其前体形式迁移速度更快，能掺入葡糖胺但不能掺入甘露糖，并且在衣霉素处理的细胞中比gD前体（pgD）具有更稳定的前体。对插入重组体和型间重组体感染的细胞进行免疫分析，将编码LP10靶抗原的基因定位到图谱单位0.892至0.924的独特短（Us）区域，不包括gD。然后，通过对使用SP6克隆制备的Us4特异性转录本进行体外翻译合成的多肽进行免疫沉淀，将LP10的靶抗原明确地定位到Us4开放阅读框。1型单纯疱疹病毒Us4基因的这种新鉴定的糖蛋白产物与先前鉴定的gB1、gC1、gE1和gH1不同。
