Richman D D, Cleveland P H, Oxman M N
J Med Virol. 1982;9(4):299-305. doi: 10.1002/jmv.1890090408.
A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus is described. It requires only a single tube culture showing viral cytopathology and can accommodate multiple specimens in a single assay. The monoclonal antibodies confer absolute specificity and the use of horseradish peroxidase-conjugated staphylococcal protein A or antiglobulin permits easy visual interpretation of the results following the 2-3 hour assay. Although it is possible that an occasional wild strain of HSV might escape recognition by monoclonal antibody, this potential problem has not been observed among the more than 500 clinical isolates tested to date, all of which have yielded an unequivocal result.
本文描述了一种使用针对单纯疱疹病毒血清型的单克隆抗体的快速酶免疫过滤技术。该技术仅需一份显示病毒细胞病理学特征的单层细胞培养物，且一次检测可容纳多个样本。单克隆抗体具有绝对特异性，使用辣根过氧化物酶标记的葡萄球菌蛋白A或抗球蛋白，使得在2 - 3小时的检测后能够轻松直观地解读结果。虽然偶尔可能会有一株野生型单纯疱疹病毒逃脱单克隆抗体的识别，但在迄今检测的500多株临床分离株中尚未发现这一潜在问题，所有这些分离株的检测结果均明确无误。
