Park Sunjung, Yang Eun Jin
Department of Clinical Research, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Evid Based Complement Alternat Med. 2018 Jun 21;2018:8580152. doi: 10.1155/2018/8580152. eCollection 2018.
Inflammation is considered a critical factor in the pathogenesis of amyotrophic lateral sclerosis (ALS). We aimed to evaluate the effect of the herbal formula Gamisoyo-San (GSS) on the muscles of hSOD1 transgenic mice, a mouse model of ALS, by examining the tissue expression of inflammation- and oxidative stress-related proteins. The mice were randomly divided into three groups: nontransgenic mice (non-Tg, n = 4), hSOD1 transgenic mice (Tg, n = 4), and GSS-treated hSOD1 transgenic mice (Tg+GSS, n = 4). Eight-week-old female hSOD1 transgenic mice were fed GSS (1 mg/g body weight) for 6 weeks. Gastrocnemius (GA) tissues were analyzed for inflammatory proteins [CD11b and toll-like receptor 4 (TLR4)] and oxidative stress-related proteins [heme oxygenase 1 (HO1) and ferritin] by western blot analysis. Administration of GSS significantly reduced the level of inflammation- and oxidative stress-related proteins in hSOD1 transgenic mice. GSS ameliorated inflammation by downregulating TLR4 and CD11b expression and regulated iron homeostasis in the GA muscle of hSOD1 mice. GSS could help reduce inflammation by regulating immune reactions in patients with ALS. To the best of our knowledge, this is the first study to demonstrate the effect of GSS on muscle inflammation in an ALS animal model.
炎症被认为是肌萎缩侧索硬化症（ALS）发病机制中的一个关键因素。我们旨在通过检测炎症和氧化应激相关蛋白的组织表达，评估中药方剂加味小续命汤（GSS）对hSOD1转基因小鼠（一种ALS小鼠模型）肌肉的影响。将小鼠随机分为三组：非转基因小鼠（非Tg，n = 4）、hSOD1转基因小鼠（Tg，n = 4）和GSS处理的hSOD1转基因小鼠（Tg+GSS，n = 4）。8周龄雌性hSOD1转基因小鼠喂食GSS（1 mg/g体重），持续6周。通过蛋白质印迹分析检测腓肠肌（GA）组织中的炎症蛋白[CD11b和Toll样受体4（TLR4）]以及氧化应激相关蛋白[血红素加氧酶1（HO1）和铁蛋白]。给予GSS可显著降低hSOD1转基因小鼠中炎症和氧化应激相关蛋白的水平。GSS通过下调TLR4和CD11b的表达改善炎症，并调节hSOD1小鼠GA肌肉中的铁稳态。GSS可能通过调节ALS患者的免疫反应来帮助减轻炎症。据我们所知，这是第一项证明GSS对ALS动物模型肌肉炎症有影响的研究。
