Sun Zhi-Fu, Gao Xing, Pinto Jayant M, He Yin, Yang QingXian, Tian Jun, Lv Qian-Wen, Wei Yong-Xiang
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Section of Otolaryngology Head and Neck Surgery, Department of Surgery, The University of Chicago, Chicago, IL 60647, USA.
World J Otorhinolaryngol Head Neck Surg. 2018 May 11;4(1):50-56. doi: 10.1016/j.wjorl.2018.03.004. eCollection 2018 Mar.
To investigate whether magnetic resonance imaging (MRI) can be used to directly assess olfactory bulb (OB) lesions and quantify the associated morphological changes of olfactory filaments (OF), also known as fila, in an OB-lesion rat model of the brain.
A surgical group ( = 5) of male Sprague-Dawley rats was subjected to the unilateral damage of the OB by a steel needle. The control group ( = 5) did not receive surgery. To assess olfactory system injury , T2-weighted MRI images were acquired in an oblique plane at a 30° angle from transverse plane one day after surgery. These brain regions were also assessed in the controls. The olfactory function was evaluated using the buried food pellet test (BFPT) 5 days before and after surgery.
The OF could be clearly observed on the MRI images from all animals. The left and right OF mean lengths (mm) were similar in the control group (0.81 ± 0.18 0.89 ± 0.17, > 0.05). In the surgical group, the OB was partially injured in all rats. These rats did not show differences in OF length between left- and right-side (0.83 ± 0.18 0.93 ± 0.24, > 0.05) at the time of measurement. The time (sec) required to find the food pellets in the BFPT was longer after than before the surgery (83.80 ± 34.37 231.44 ± 53.23, < 0.05).
MicroMRI may be a feasible tool to evaluate the OF and OBs in rat models. The unilateral partial OB lesion model appears to be an effective post-traumatic olfactory dysfunction model.
在大鼠脑嗅球损伤模型中，研究磁共振成像（MRI）是否可用于直接评估嗅球（OB）病变，并量化嗅丝（OF，也称为fila）的相关形态学变化。
雄性Sprague-Dawley大鼠手术组（n = 5）通过钢针造成单侧嗅球损伤。对照组（n = 5）未接受手术。为评估嗅觉系统损伤，术后1天在与横断面成30°角的斜平面上采集T2加权MRI图像。对照组也对这些脑区进行评估。在手术前后5天使用埋食颗粒试验（BFPT）评估嗅觉功能。
所有动物的MRI图像上均可清晰观察到嗅丝。对照组左右嗅丝平均长度（mm）相似（0.81±0.18对0.89±0.17，P>0.05）。手术组中，所有大鼠的嗅球均受到部分损伤。测量时，这些大鼠左右侧嗅丝长度无差异（0.83±0.18对0.93±0.24，P>0.05）。与手术前相比，BFPT中找到食物颗粒所需时间（秒）在术后更长（83.80±34.37对231.44±53.23，P<0.05）。
显微MRI可能是评估大鼠模型中嗅丝和嗅球的可行工具。单侧部分嗅球损伤模型似乎是一种有效的创伤后嗅觉功能障碍模型。
