The Hudson Institute of Medical Research, Clayton, Victoria, Australia.
Department of Molecular and Translational Medicine, Monash University, Clayton, Victoria, Australia.
FASEB J. 2019 Jan;33(1):584-605. doi: 10.1096/fj.201800086R. Epub 2018 Jul 23.
Repair after damage is essential for tissue homeostasis. Postmenstrual endometrial repair is a cyclical manifestation of rapid, scar-free, tissue repair taking ∼3-5 d. Skin repair after wounding is slower (∼2 wk). In the case of chronic wounds, it takes months to years to restore integrity. Herein, the unique "rapid-repair" endometrial environment is translated to the "slower repair" skin environment. Menstrual fluid (MF), the milieu of postmenstrual endometrial repair, facilitates healing of endometrial and keratinocyte "wounds" in vitro, promoting cellular adhesion and migration, stimulates keratinocyte migration in an ex vivo human skin reconstruct model, and promotes re-epithelialization in an in vivo porcine wound model. Proteomic analysis of MF identified a large number of proteins: migration inhibitory factor, neutrophil gelatinase-associated lipocalin, follistatin like-1, chemokine ligand-20, and secretory leukocyte protease inhibitor were selected for further investigation. Functionally, they promote repair of endometrial and keratinocyte wounds by promoting migration. Translation of these and other MF factors into a migration-inducing treatment paradigm could provide novel treatments for tissue repair.-Evans, J., Infusini, G., McGovern, J., Cuttle, L., Webb, A., Nebl, T., Milla, L., Kimble, R., Kempf, M., Andrews, C. J., Leavesley, D., Salamonsen, L. A. Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair.
损伤后的修复对于组织稳态至关重要。月经后子宫内膜修复是一种快速、无疤痕、组织修复的周期性表现，大约需要 3-5 天。皮肤损伤后的修复较慢（约 2 周）。在慢性伤口的情况下，需要数月甚至数年才能恢复完整性。在此，独特的“快速修复”子宫内膜环境被转化为“较慢修复”的皮肤环境。月经血（menstrual fluid，MF）是月经后子宫内膜修复的环境，促进子宫内膜和角质形成细胞“伤口”的愈合，在体外促进细胞黏附和迁移，在体外人类皮肤重建模型中刺激角质形成细胞迁移，并在体内猪伤口模型中促进再上皮化。MF 的蛋白质组分析鉴定出大量蛋白质：迁移抑制因子、中性粒细胞明胶酶相关脂质运载蛋白、卵泡抑素样 1、趋化因子配体 20 和分泌白细胞蛋白酶抑制剂被选为进一步研究的对象。这些和其他 MF 因子的功能是通过促进迁移来促进子宫内膜和角质形成细胞伤口的修复。将这些和其他 MF 因子转化为诱导迁移的治疗方案可能为组织修复提供新的治疗方法。
