Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (ICBS/UFRGS), Porto Alegre, RS, Brazil; Instituto Nacional de Pesquisa em Resistência Antimicrobiana (INPRA), Rio Grande do Sul, Brazil.
Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
J Glob Antimicrob Resist. 2018 Sep;14:275-276. doi: 10.1016/j.jgar.2018.07.007. Epub 2018 Jul 20.
Pseudomonas aeruginosa strains L25 and M12 were isolated from hospital effluent in southern Brazil.
The whole genomes of the isolates were sequenced using an Illumina MiSeq system. The data were analysed using SPAdes, Prokka and Geneious, and antimicrobial resistance genes were predicted using ResFinder. PubMLST protocols were used to define the sequence type (ST).
Many multidrug efflux pump systems as well as various antimicrobial resistance genes were identified in the two P. aeruginosa strains. The strains were identified as ST2963, a novel carbapenem-resistant sequence type.
Here we describe the genome sequences of two carbapenem-resistant P. aeruginosa strains and characterised a novel sequence type (ST2963).
假单胞菌菌株 L25 和 M12 从巴西南部医院污水中分离得到。
使用 Illumina MiSeq 系统对分离株的全基因组进行测序。使用 SPAdes、Prokka 和 Geneious 对数据进行分析，并使用 ResFinder 预测抗生素耐药基因。使用 PubMLST 方案定义序列型 (ST)。
在这两种铜绿假单胞菌菌株中发现了许多多药外排泵系统和各种抗生素耐药基因。这些菌株被鉴定为 ST2963，这是一种新型耐碳青霉烯序列型。
本研究描述了两株耐碳青霉烯铜绿假单胞菌的基因组序列，并对一种新型序列型 (ST2963) 进行了特征分析。
