Greifová Gabriela, Body Patrik, Greif Gabriel, Greifová Maria, Dubničková Martina
Comenius University, Faculty of Pharmacy, Department of Cellular and Molecular Biology of Drugs, Odbojárov 10, 832 32 Bratislava, Slovak Republic.
Slovak University of Technology, Faculty of Chemical and Food Technology, Department of Food Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic.
Immunobiology. 2018 Nov;223(11):618-626. doi: 10.1016/j.imbio.2018.07.007. Epub 2018 Jul 20.
Histamine derived from lactobacilli isolates is considered to be a potential immunomodulator able to interact with the host immune system. We tested the effect of pure histamine (0.413 mM) together with the effect of cell-culture supernatants (CCS) containing different concentration of histamine produced by two of Lactobacillus reuteri isolates on the activities of antioxidant enzyme, as well as on the phagocytic activity of human leucocytes (HL). Phagocytic activity represents the non-specific immune response of HL homogenate, in vitro. Analysed histamine-producers were represented by a goatling isolate named L. reuteri KO5 and a lamb isolate named L. reuteri E and histamine production was determined using HPLC method connected with UV detection. Concretely, the samples contained the mixture of isolated HL and the addition of lactobacilli CCS at three different final concentrations of histamine ∼ 0.1, 1.8 and 5.4 mM. It was found that pure histamine (0.413 mM) did not significantly influence the oxidant-antioxidant balance in HL demonstrated by unchanged degree of HL lipid peroxidation. However, at the same time, the final activity of catalase and superoxide dismutase were significantly changed (p ≤ 0.001). Moreover, the phagocytic index (p ≤ 0.01), lysozyme (p ≤ 0.05) and peroxidase activity (p ≤ 0.001), and production of IL-1β significantly decreased. CCS containing different concentration of produced histamine were also able to modulate the host non-specific immune response together with the enzymatic activity of SOD and catalase too. However, our findings indicated that the impact of lactobacilli histamine is strictly strain-dependent and concentration dependent. Moreover, it seems that histamine is not the only one lactobacilli metabolite, which may play an important role in overall immunomodulatory and antioxidant potential of tested lactobacilli.
源自罗伊氏乳杆菌分离株的组胺被认为是一种能够与宿主免疫系统相互作用的潜在免疫调节剂。我们测试了纯组胺（0.413 mM）的作用，以及两种罗伊氏乳杆菌分离株产生的含有不同浓度组胺的细胞培养上清液（CCS）对抗氧化酶活性以及人白细胞（HL）吞噬活性的影响。吞噬活性代表了HL匀浆在体外的非特异性免疫反应。所分析的组胺产生菌以一株名为罗伊氏乳杆菌KO5的山羊分离株和一株名为罗伊氏乳杆菌E的羔羊分离株为代表，组胺产量通过与紫外检测联用的高效液相色谱法测定。具体而言，样品包含分离的HL混合物以及添加了三种不同终浓度组胺（约0.1、1.8和5.4 mM）的罗伊氏乳杆菌CCS。结果发现，纯组胺（0.413 mM）并未显著影响HL中的氧化还原平衡，这通过HL脂质过氧化程度未变得以证明。然而，与此同时，过氧化氢酶和超氧化物歧化酶的终活性发生了显著变化（p≤0.001）。此外，吞噬指数（p≤0.01）、溶菌酶（p≤0.05）和过氧化物酶活性（p≤0.001）以及IL-1β的产生均显著降低。含有不同浓度产生的组胺的CCS也能够调节宿主的非特异性免疫反应以及SOD和过氧化氢酶的酶活性。然而，我们的研究结果表明，乳杆菌组胺的影响严格依赖于菌株和浓度。此外，组胺似乎不是唯一一种可能在测试乳杆菌的整体免疫调节和抗氧化潜力中发挥重要作用的乳杆菌代谢产物。
