Institute of Dentistry, University of Turku, Turku, Finland.
Curr Microbiol. 2011 Feb;62(2):618-22. doi: 10.1007/s00284-010-9752-9. Epub 2010 Sep 11.
In clinical studies, probiotic bacteria have decreased the counts of salivary mutans streptococci (MS). We compared the effects of probiotic Lactobacillus strains on the biofilm formation of Streptococcus mutans. The bacterial strains used included four S. mutans strains (reference strains NCTC 10449 and Ingbritt and clinical isolates 2366 and 195) and probiotic strains Lactobacillus rhamnosus GG, L. plantarum 299v, and L. reuteri strains PTA 5289 and SD2112. The ability of MS to adhere and grow on a glass surface, reflecting biofilm formation, was studied in the presence of the lactobacilli (LB). The effect of LB culture supernatants on the viability of the MS was studied as well. All of the LB inhibited the biofilm formation of the clinical isolates of MS (P < 0.001). The biofilm formation of the reference strains of MS was also inhibited by the LB, but L. plantarum and L. reuteri PTA 5289 showed a weaker inhibition when compared to L. reuteri SD2112 and L. rhamnosus GG. Viable S. mutans cells could be detected in the biofilms and culture media only when the experiments were performed with the L. reuteri strains. The L. reuteri strains were less efficient in killing the MS also in the tests performed with the culture supernatants. The pHs of the supernatants of L. reuteri were higher compared to those of L. rhamnosus GG and L. plantarum; P < 0.001. In conclusion, our results demonstrated that four commonly used probiotics interfered with S. mutans biofilm formation in vitro, and that the antimicrobial activity against S. mutans was pH-dependent.
在临床研究中，益生菌可减少唾液变形链球菌（MS）的数量。我们比较了益生菌乳杆菌菌株对变形链球菌生物膜形成的影响。使用的细菌菌株包括四种变形链球菌菌株（参考菌株 NCTC 10449 和 Ingbritt 以及临床分离株 2366 和 195）和益生菌菌株鼠李糖乳杆菌 GG、植物乳杆菌 299v 和乳杆菌菌株 PTA 5289 和 SD2112。在存在乳杆菌（LB）的情况下，研究了 MS 粘附和在玻璃表面生长的能力，反映了生物膜的形成。还研究了 LB 培养上清液对 MS 活力的影响。所有 LB 均抑制了 MS 临床分离株的生物膜形成（P <0.001）。MS 的参考菌株的生物膜形成也被 LB 抑制，但与 L. reuteri SD2112 和 L. rhamnosus GG 相比，植物乳杆菌和 L. reuteri PTA 5289 的抑制作用较弱。只有当实验使用 L. reuteri 菌株进行时，才能在生物膜和培养基中检测到存活的 S. mutans 细胞。在使用培养上清液进行的测试中，L. reuteri 菌株对 MS 的杀菌效率也较低。与 L. rhamnosus GG 和 L. plantarum 相比，L. reuteri 菌株的上清液 pH 值更高；P <0.001。总之，我们的结果表明，四种常用的益生菌在体外干扰了 S. mutans 生物膜的形成，并且对 S. mutans 的抗菌活性依赖于 pH 值。
