Greifová Gabriela, Májeková Hyacinta, Greif Gabriel, Body Patrik, Greifová Maria, Dubničková Martina
Department of Cellular and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovak Republic.
Faculty of Chemical and Food Technology, Department of Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovak Republic.
Folia Microbiol (Praha). 2017 Nov;62(6):515-524. doi: 10.1007/s12223-017-0524-9. Epub 2017 Apr 11.
Antimicrobial and immunomodulatory potential of various Lactobacillus reuteri strains is closely connected to their metabolite production profile under given cultivation conditions. We determined the in vitro production of antimicrobial substances such as organic acids, ethanol, and reuterin by four strains of L. reuteri (L. reuteri E, L. reuteri KO5, L. reuteri CCM 3625, and L. reuteri ATCC 55730). All studied L. reuteri strains showed the ability to produce lactic acid, acetic acid, and ethanol with concominant consumption of glucose and together with phenyllactic acid-a potent antifungal compound-with concominant consumption of phenylalanine. The reuterin production from glycerol was confirmed for all analyzed lactobacilli strains except L. reuteri CCM 3625. Production of organic acids, ethanol, and reuterin is significantly involved in antimicrobial activity of lactobacilli which was determined using the dual-culture overlay diffusion method against six indicator bacteria and five indicator moulds. In comparison to the referential L. reuteri ATCC 55730, the highest inhibition potential was observed against Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 3955. Among analyzed indicators of moulds, the growth of Alternaria alternata CCM F-128 was the most inhibited by all four analyzed L. reuteri strains. Finally, the immunomodulatory potential of analyzed lactobacilli were proven by the determination of the in vitro production of biogenic amines histamine and tyramine. L. reuteri CCM 3625 was able to produce tyramine, and L. reuteri E and L. reuteri KO5 were able to produce histamine under given cultivation conditions.
在特定培养条件下，不同罗伊氏乳杆菌菌株的抗菌和免疫调节潜力与其代谢产物生成谱密切相关。我们测定了四株罗伊氏乳杆菌（罗伊氏乳杆菌E、罗伊氏乳杆菌KO5、罗伊氏乳杆菌CCM 3625和罗伊氏乳杆菌ATCC 55730）体外抗菌物质如有机酸、乙醇和罗伊氏菌素的生成情况。所有研究的罗伊氏乳杆菌菌株都显示出能够产生乳酸、乙酸和乙醇，同时消耗葡萄糖，以及产生苯乳酸（一种有效的抗真菌化合物）并同时消耗苯丙氨酸的能力。除罗伊氏乳杆菌CCM 3625外，所有分析的乳酸杆菌菌株均证实能从甘油中产生罗伊氏菌素。使用双培养平板扩散法针对六种指示细菌和五种指示霉菌测定发现，有机酸、乙醇和罗伊氏菌素的产生与乳酸杆菌的抗菌活性密切相关。与参考菌株罗伊氏乳杆菌ATCC 55730相比，对大肠杆菌CCM 3988和铜绿假单胞菌CCM 3955观察到最高的抑制潜力。在分析的霉菌指示菌中，所有四株分析的罗伊氏乳杆菌菌株对链格孢CCM F - 128的生长抑制作用最强。最后，通过测定生物胺组胺和酪胺的体外生成情况，证明了所分析乳酸杆菌的免疫调节潜力。在特定培养条件下，罗伊氏乳杆菌CCM 3625能够产生酪胺，罗伊氏乳杆菌E和罗伊氏乳杆菌KO5能够产生组胺。
