Elboim C M, Reinisch C L, Schlossman S F
J Immunol. 1977 Mar;118(3):1042-8.
The migration of splenic T and B lymphocytes into syngeneic tumors undergoing immunologic rejection was investigates. Spleen cells were obtained from normal BALC/c mice or BALB/c mice bearing tumors induced by murine sarcoma virus (MSV). Either whole spleen cells or immunoabsorbent purified T and B cells were radiolabeled with sodium chromate-51 and injected i.v. into normal or MSV inducted-tumor bearing syngeneic recipients. Twenty-four hours later the recipient mice were sacrificed and radioactivity was assessed for tumor, contralateral normal muscle, the lymph nodes draining the tumor and contralateral draining lymph nodes, peripheral lymph nodes, spleen, and liver. Both T and B lymphocytes from either normal or MSV tumor-bearing animals show greatly increased migration into the tumor when compared with normal muscle. Migration of T cells from both normal and MSV tumor bearers was 30 times that of migration to normal muscle. B cells from tumor-bearing mice, on the other hand, localized in the tumor itself only 50% as frequently as did B cells from normal animals. In addition, T cells from MSV tumor bearers were found in the highest proportion in the lymph node draining the tumor site. We conclude that T and B lymphocytes from either normal or tumor-bearing mice migrate to a syngeneic tumor undergoing immunologic rejection. In contrast, the migration of both T and B cells from tumor-bearing animals was decreased to the peripheral lymph nodes at the time of maximum tumor growth.
研究了脾T淋巴细胞和B淋巴细胞向正在经历免疫排斥的同基因肿瘤的迁移情况。脾细胞取自正常BALB/c小鼠或携带鼠肉瘤病毒（MSV）诱导肿瘤的BALB/c小鼠。将全脾细胞或免疫吸附纯化的T细胞和B细胞用51铬酸钠进行放射性标记，然后静脉注射到正常或MSV诱导肿瘤的同基因受体小鼠体内。24小时后，处死受体小鼠，评估肿瘤、对侧正常肌肉、引流肿瘤的淋巴结和对侧引流淋巴结、外周淋巴结、脾脏和肝脏中的放射性。与正常肌肉相比，来自正常或MSV荷瘤动物的T淋巴细胞和B淋巴细胞向肿瘤的迁移均显著增加。正常和MSV荷瘤小鼠的T细胞迁移到肿瘤的量是迁移到正常肌肉量的30倍。另一方面，荷瘤小鼠的B细胞定位在肿瘤本身的频率仅为正常动物B细胞的50%。此外，在引流肿瘤部位的淋巴结中发现来自MSV荷瘤小鼠的T细胞比例最高。我们得出结论，来自正常或荷瘤小鼠的T淋巴细胞和B淋巴细胞会迁移到正在经历免疫排斥的同基因肿瘤中。相比之下，在肿瘤生长达到最大值时，来自荷瘤动物的T细胞和B细胞向外周淋巴结的迁移均减少。
