Hapten-specific leukocyte migration inhibition. I. Inhibition of cells from animals immunized with DNP-KLH by epsilon-DNP-L-lysine Ficoll.

Guiena pigs were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH) or with hemocyanin in complete Freud's adjevant. The migration of oil-induced peritoneal exudate cells was measured in the presence and absence of epsilon-dinitrophenyl-lysine-tyrosinyl Ficoll (DNPL-F). When the cells came from animals immunized with DNP-KLH their migration was inhibited by DNPL-F. Control cells from animals immunized with KLH or not immunized migrated normally in the presence of DNPL-F.