Amlot P L, Hayes A E, Gray D, Gordon-Smith E C, Humphrey J H
Clin Exp Immunol. 1986 Apr;64(1):125-35.
Thymus-independent (TI) and thymus-dependent (TD) primary immune responses were measured in 67 controls and 13 bone marrow transplant (BMT) recipients treated with cyclosporine (CSP) by immunizing with a synthetic antigen (DNP-Ficoll) and keyhole limpet haemocyanin (KLH). DNP-Ficoll induced similar TI antibody responses in controls and BMT recipients except that antibody levels declined much more rapidly in BMT recipients. The IgM and IgG antibodies induced by DNP-Ficoll only recognized the DNP epitope and not the Ficoll carrier. Both IgM and IgG classes of antibody showed similar TI behaviour upon immunization and re-immunization. The antibodies to DNP-Ficoll were overwhelmingly of the IgG1 subclass. The TD response to KLH evoked both delayed hypersensitivity (DH) and antibody production. DH developed at the site of immunization in 68% of controls and in 88% upon subsequent challenge with KLH. None of the BMT recipients on CSP developed DH. KLH antibody arose in 88% of controls but in only one BMT recipient on CSP. Eight BMT recipients were re-immunized with KLH 2-6 weeks after stopping CSP and only one made primary DH and antibody responses, arguing that CSP inhibited priming as well as any detectable response to KLH. The immunization procedure described has proved a sensitive and comprehensive method of quantitating human immune responses in vivo and is readily adaptable for in vitro studies.
通过用合成抗原（二硝基苯 - 聚蔗糖，DNP-Ficoll）和钥孔戚血蓝蛋白（KLH）免疫，在67名对照者和13名接受环孢素（CSP）治疗的骨髓移植（BMT）受者中检测了非胸腺依赖性（TI）和胸腺依赖性（TD）初级免疫反应。DNP-Ficoll在对照者和BMT受者中诱导了相似的TI抗体反应，只是BMT受者中的抗体水平下降得更快。DNP-Ficoll诱导的IgM和IgG抗体仅识别DNP表位，而不识别聚蔗糖载体。在免疫和再次免疫时，IgM和IgG两类抗体均表现出相似的TI行为。针对DNP-Ficoll的抗体绝大多数是IgG1亚类。对KLH的TD反应引发了迟发型超敏反应（DH）和抗体产生。68%的对照者在免疫部位出现DH，在用KLH再次攻击后，这一比例为88%。接受CSP治疗的BMT受者均未出现DH。88%的对照者产生了KLH抗体，但接受CSP治疗的BMT受者中只有一人产生了该抗体。8名BMT受者在停止使用CSP 2 - 6周后用KLH再次免疫，只有一人产生了初级DH和抗体反应，这表明CSP抑制了启动以及对KLH的任何可检测到的反应。所描述的免疫程序已被证明是一种在体内定量人类免疫反应的灵敏且全面的方法，并且很容易适用于体外研究。
