Ben Halima Meriam, Kallel Amani, Baara Abir, Ben Wafi Safa, Sanhagi Haifa, Slimane Hedia, Jemaa Riadh, Feki Moncef
a Laboratory of Biochemistry, Faculty of Medicine of Tunis , University of Tunis El Manar, Rabta Hospital , Tunis , Tunisia.
b Faculty of Sciences of Tunis , University of Tunis El Manar , Tunis , Tunisia.
Biomarkers. 2018 Dec;23(8):787-792. doi: 10.1080/1354750X.2018.1499129. Epub 2018 Aug 23.
Variations in the fat mass and obesity-associated gene (FTO) has been associated with obesity in many populations, but the results are conflicting.
The aim of this study was to evaluate the effect of the rs9939609 polymorphism in the FTO gene on obesity risk and plasma leptin, adiponectin, insulin and lipid concentrations in Tunisians.
Four hundred and ninety-four subjects with obesity and 334 non-obese participated in this study. The rs9939609 (T/A) genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Significant differences in genotype frequencies were observed between cases and controls. In the separate analysis by gender, the association between the AA genotype and obesity was statistically significant in women but not in men. After stratification by obesity class this association remains only with obesity class III.
Our study is in agreement with studies on Caucasian, Portuguese and Cebu Filipino populations where a gender-specific association was found between rs9939609 polymorphism and obesity. It is also in agreement with studies on Mexican, Spanish and European populations, where an association was found with obesity class III.
The rs9939609 polymorphism of FTO gene is associated with obesity, especially obesity class III in women.
脂肪量和肥胖相关基因（FTO）的变异在许多人群中都与肥胖有关，但结果相互矛盾。
本研究旨在评估突尼斯人FTO基因中rs9939609多态性对肥胖风险以及血浆瘦素、脂联素、胰岛素和脂质浓度的影响。
494名肥胖受试者和334名非肥胖受试者参与了本研究。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法测定rs9939609（T/A）基因型。
病例组和对照组之间观察到基因型频率存在显著差异。按性别进行单独分析时，AA基因型与肥胖之间的关联在女性中具有统计学意义，而在男性中则无。按肥胖等级分层后，这种关联仅与III级肥胖有关。
我们的研究与对白种人、葡萄牙人和宿务菲律宾人群的研究一致，这些研究发现rs9939609多态性与肥胖之间存在性别特异性关联。它也与对墨西哥、西班牙和欧洲人群的研究一致，这些研究发现与III级肥胖有关。
FTO基因的rs9939609多态性与肥胖有关，尤其是与女性的III级肥胖有关。
