Daya Mulianah, Pujianto Dwi Ari, Witjaksono Fiastuti, Priliani Lidwina, Susanto Jimmy, Lukito Widjaja, Malik Safarina G
Department of Nutrition, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.
Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesiaa.
Asia Pac J Clin Nutr. 2019;28(1):183-191. doi: 10.6133/apjcn.201903_28(1).0024.
Data suggest that genetic factors are associated with BMI. The fat mass and obesity- associated (FTO) gene modulates adipogenesis through alternative splicing and m6A demethylation. Individuals with FTO rs9939609 gene polymorphism have a preference for energy-dense foods. This study investigates the relationship between FTO rs9939609 and obesity and preference for dietary fat intake among selected Indonesian adults.
A total of 40 non-obese and 40 obese participants aged 19-59 living in Jakarta were recruited. Body composition measurements included body weight, height, BMI, waist circumference, and body fat mass. Dietary intake was assessed using a semiquantitative food frequency questionnaire and food recall over 2 × 24-h periods. Genetic variation was determined using amplification-refractory mutation system polymerase chain reaction.
The genotype distribution of the FTO gene (rs9939609) was at Hardy- Weinberg equilibrium (p=1) with minor allele frequency=0.19. Individuals with AT/AA genotypes had 3.72 times higher risk of obesity (p=0.009) and 5.98 times higher dietary fat intake (p=0.02) than those with TT genotype. Obese participants with the AT/AA genotypes had 1.40 times higher dietary fat intake than those with the TT genotype (p=0.016).
These findings suggest that Indonesian adults with AT/AA genotypes of the FTO rs9939609 have higher obesity risks and preferences for high dietary fat intake than those with TT genotype.
数据表明遗传因素与体重指数（BMI）相关。脂肪量和肥胖相关（FTO）基因通过可变剪接和m6A去甲基化调节脂肪生成。携带FTO rs9939609基因多态性的个体偏好能量密集型食物。本研究调查了FTO rs9939609与肥胖之间的关系，以及在选定的印度尼西亚成年人中对膳食脂肪摄入的偏好。
招募了居住在雅加达的40名19 - 59岁的非肥胖参与者和40名肥胖参与者。身体成分测量包括体重、身高、BMI、腰围和体脂肪量。使用半定量食物频率问卷和2个24小时时间段的食物回忆法评估膳食摄入量。使用扩增阻滞突变系统聚合酶链反应确定基因变异。
FTO基因（rs9939609）的基因型分布处于哈迪 - 温伯格平衡（p = 1），次要等位基因频率 = 0.19。与TT基因型个体相比，携带AT/AA基因型的个体肥胖风险高3.72倍（p = 0.009），膳食脂肪摄入量高5.98倍（p = 0.02）。携带AT/AA基因型的肥胖参与者的膳食脂肪摄入量比携带TT基因型的参与者高1.40倍（p = 0.016）。
这些发现表明，与TT基因型个体相比，携带FTO rs9939609基因AT/AA基因型的印度尼西亚成年人肥胖风险更高，且更偏好高膳食脂肪摄入。
