Cardoso Glêbia Alexa, Persuhn Darlene Camati, Ribeiro Mateus Duarte, de Sousa Bruno Rafael Virgínio, Sena Klécia de Farias, de Almeida Antônio Eduardo Monteiro, Modesto-Filho João, da Silva Raquel Suelen Brito, Silva Alexandre Sérgio
Laboratory of Applied Studies in Physical Training to Performance and Health - LETFADS, Department of Physical Education, Federal University of Paraíba, João Pessoa, Brazil.
Associate Graduate Program in Physical Education - UPE/UFPB, Department of Physical Education, Federal University of Paraíba, João Pessoa, Brazil.
Front Physiol. 2020 May 8;11:385. doi: 10.3389/fphys.2020.00385. eCollection 2020.
The objective of this study was to verify the influence of the Pro12Ala polymorphism of the PPARγ2 gene in response of a training program on the body composition. Sixty-nine previously inactive men and women (32.8 ± 8.2 years) were genotyped and underwent a 12-week aerobic (running/walking) training program (3-5 sessions, 40 - 60 min per session, and intensity between the aerobic and anaerobic threshold) (experimental group = 53) or were part of the control group ( = 16). They were tested for aerobic capacity (ergospirometry), body composition (DXA), abdomen, waist and hip circumferences and nutritional assessment before and 48 h after the experimental protocol. Two-way repeated measures ANOVA test was used to verify possible differences in variables between the experimental vs. control groups or Pro/Pro vs. Pro/Ala groups, and the Chi-squared test was used to verify the distribution of responders and non-responders according to genotype ( < 0.05). Frequencies of 75.5% Pro/Pro ( = 40) and 24.5% Pro/Ala ( = 13) were found, without any occurrence of the recessive homozygote. Body fat reduction was initially confirmed compared to a control group which did not exercise ( = 16; 29.1 ± 8.8 years), so that the exercise group obtained a reduction of -1.3 kg vs. -0.3 kg in the control group ( = 0.03). When they were divided by genotype, there were significant changes in fat mass (-1.3 ± 2.1 kg; = 0.00), lean mass (0.6 ± 1.5 kg; = 0.02), fat percentage (-1.3 ± 1.6; = 0.00), waist circumference (-2.2 ± 2.9 cm; = 0.00), abdomen circumference (-3.3 ± 3.6 cm; = 0.00) and hip circumference (-2.7 ± 2.7 cm; = 0.00) for Pro/Pro genotypes; and fat mass (-1.1 ± 1.7 kg; = 0.04), fat percentage (-0.9 ± 1.5; = 0.04), abdomen circumference (-3.9 ± 3.5 cm; = 0.00) and hip circumference (-1.8 ± 1.8 cm; = 0.00) for Pro/Ala genotypes, without any group interaction differences. The Chi squared test revealed no differences in the distribution of responders or non-responders according to genotype. It is concluded that an aerobic training program promotes weight loss, but the Pro12Ala polymorphism in the PPARγ2 gene does not influence the variability of aerobic-induced exercise weight loss.
本研究的目的是验证PPARγ2基因Pro12Ala多态性对一项训练计划作用于身体成分的影响。对69名先前不运动的男性和女性（32.8±8.2岁）进行基因分型，并让他们参加一个为期12周的有氧（跑步/步行）训练计划（每周3 - 5次，每次40 - 60分钟，强度介于有氧和无氧阈值之间）（实验组 = 53人），或作为对照组（ = 16人）。在实验方案实施前和实施后48小时，对他们进行有氧能力（运动心肺功能测试）、身体成分（双能X线吸收法）、腹部、腰围和臀围以及营养评估测试。采用双向重复测量方差分析来验证实验组与对照组之间或Pro/Pro与Pro/Ala组之间变量的可能差异，采用卡方检验来验证根据基因型划分的反应者和无反应者的分布情况（ < 0.05）。发现Pro/Pro基因型频率为75.5%（ = 40），Pro/Ala基因型频率为24.5%（ = 13），未出现隐性纯合子。与未运动的对照组（ = 16人；29.1±8.8岁）相比，最初证实运动组身体脂肪减少，运动组减少了-1.3 kg，而对照组减少了-0.3 kg（ = 0.03）。当按基因型划分时，Pro/Pro基因型在脂肪量（-1.3±2.1 kg； = 0.00）、去脂体重（0.6±1.5 kg； = 0.02）、脂肪百分比（-1.3±1.6； = 0.00）、腰围（-2.2±2.9 cm； = 0.00）、腹围（-3.3±3.6 cm； = 0.00）和臀围（-2.7±2.7 cm； = 0.00）方面有显著变化；Pro/Ala基因型在脂肪量（-1.1±1.7 kg； = 0.04）、脂肪百分比（-0.9±1.5； = 0.04）、腹围（-3.9±3.5 cm； = 0.00）和臀围（-1.8±1.8 cm； = 0.00）方面有显著变化，且无任何组间交互差异。卡方检验显示根据基因型划分的反应者或无反应者的分布没有差异。得出的结论是，有氧训练计划可促进体重减轻，但PPARγ2基因中的Pro12Ala多态性不影响有氧运动引起的体重减轻的变异性。
