Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S4K1, Canada.
School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China.
Angew Chem Int Ed Engl. 2018 Sep 17;57(38):12440-12443. doi: 10.1002/anie.201806489. Epub 2018 Aug 21.
Micrometer-sized functional nucleic acid (FNA) superstructures (denoted as 3D DNA) were examined as a unique class of biorecognition elements to produce highly functional bioactive paper surfaces. 3D DNA containing repeating sequences of either a DNA aptamer or DNAzyme was created from long-chain products of rolling circle amplification followed by salt aging. The resulting 3D DNA retained its original spherical shape upon inkjet printing and adhered strongly to the paper surface via physisorption. 3D DNA paper sensors showed resistance to degradation by nucleases, suppressed nonspecific protein adsorption, and provided a much higher surface density of functional DNA relative to monomeric FNAs, making such species ideally suited for development of paper-based biosensors.
微米级功能核酸（FNA）超结构（表示为 3D DNA）被用作一类独特的生物识别元件，以产生具有高功能的生物活性纸表面。3D DNA 含有 DNA 适体或 DNA 酶的重复序列，由滚环扩增的长链产物制成，然后进行盐老化。所得的 3D DNA 在喷墨打印时保留其原始的球形形状，并通过物理吸附牢固地附着在纸表面上。3D DNA 纸传感器显示出对核酸酶降解的抗性、抑制非特异性蛋白质吸附，并提供比单体 FNA 更高的功能 DNA 表面密度，使得此类物质非常适合开发基于纸张的生物传感器。
