mRNA 驱动急性心肌梗死中的靶向基因传递。
MRNA Drives Targeted Gene Delivery in Acute Myocardial Infarction.
机构信息
1 Department of Cardiovascular Medicine, Mayo Clinic, Rochester, Minnesota.
2 VanCleve Cardiac Regenerative Medicine Program, Center for Regenerative Medicine, Mayo Clinic, Rochester, Minnesota.
出版信息
Tissue Eng Part A. 2019 Jan;25(1-2):145-158. doi: 10.1089/ten.TEA.2017.0445. Epub 2018 Sep 21.
Abstract
The MRNA (microencapsulated modified messenger RNA) platform is an approach to deliver messenger RNA (mRNA) in vivo, achieving a nonintegrating and viral-free approach to gene therapy. This technology was, in this study, tested for its utility in the myocardium, providing a unique avenue for targeted gene delivery into the freshly infarcted myocardial tissue. This study provides the evidentiary basis for the use of MRNA in the heart through depiction of its performance in cultured cells, healthy rodent myocardium, and acutely injured porcine hearts. By testing the technology in large animal models of infarction, compatibility of MRNA with current coronary intervention procedures was verified.
摘要
mRNA（微囊化修饰信使 RNA）平台是一种在体内递送信使 RNA（mRNA）的方法，实现了非整合和无病毒的基因治疗方法。这项技术在本研究中被测试了其在心肌中的效用，为将靶向基因递送到新鲜梗死的心肌组织中提供了独特的途径。本研究通过描绘其在培养细胞、健康啮齿动物心肌和急性损伤的猪心中的性能，为 mRNA 在心脏中的应用提供了证据基础。通过在心肌梗死的大动物模型中测试该技术，验证了 mRNA 与当前冠状动脉介入程序的兼容性。
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