Persson U, Coutinho A, Möller G
Scand J Immunol. 1977;6(1-2):15-30. doi: 10.1111/j.1365-3083.1977.tb00319.x.
Supernatants from incubated normal mouse spleen cells suppressed the DNA synthetic response induced by polyclonal B- and T-cell activators and the primary immune response to sheep erythrocytes in normal spleen cells in vitro. The inhibitory effects of the supernatants were found to be dependent on the culture system used. The main cell population producing or releasing the inhibitory factors was nonadherent spleen cells, but macrophages and bone marrow cells could also give supernatants with inhibitory effects. Thymocytes did not release any inhibitors under the same conditions. Separation of the supernatants showed that there were at least two factors with inhibitory effects on proliferation in lymphocytes. One of these factors had a molecular weight of less than 10,000 (not degradable by protease) and suppressed activation by all tested polyclonal B- and T-cell activators. Another factor (a protein with a molecular weight of more than 30,000) inhibited activation by some polyclonal B-cell activators (lipopolysaccharide, type III pneumococcal polysaccharide) whereas the proliferation induced by dextran sulfate was less or not at all affected under the same culture conditions. Trivial mechanisms for inhibition by supernatant factors, such as medium depletion, general toxicity, and accumulation of free thymidine in the medium, were excluded.
来自培养的正常小鼠脾细胞的上清液，在体外可抑制多克隆B细胞和T细胞激活剂诱导的DNA合成反应，以及正常脾细胞对绵羊红细胞的初次免疫反应。发现上清液的抑制作用取决于所使用的培养系统。产生或释放抑制因子的主要细胞群体是非黏附性脾细胞，但巨噬细胞和骨髓细胞也可产生具有抑制作用的上清液。在相同条件下，胸腺细胞不释放任何抑制剂。对上清液进行分离后发现，至少有两种因子对淋巴细胞增殖具有抑制作用。其中一种因子的分子量小于10,000（不能被蛋白酶降解），可抑制所有测试的多克隆B细胞和T细胞激活剂的激活作用。另一种因子（一种分子量大于30,000的蛋白质）可抑制某些多克隆B细胞激活剂（脂多糖、Ⅲ型肺炎球菌多糖）的激活作用，而在相同培养条件下，硫酸葡聚糖诱导的增殖受到的影响较小或根本不受影响。排除了上清液因子抑制作用的一些微不足道的机制，如培养基耗尽、一般毒性以及培养基中游离胸苷的积累。
