Faculty of Metallurgy and Technology, University of Montenegro, Dzordza Vasingtona bb, 20000 Podgorica, Montenegro.
Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11001 Belgrade, Serbia.
Molecules. 2018 Jul 26;23(8):1864. doi: 10.3390/molecules23081864.
The aim of this study was to establish correlation of chemical composition and antioxidant activity of bilberry plants from Montenegro. Total phenolic, tannin, flavonoid, procyanidin and anthocyanin contents were determined in fruits and leaves extracts using spectrophotometric methods, while the measurements of metal content was carried out in an Inductively Coupled Atomic Emission Spectrometer. Qualitative and quantitative analyses of major phenolics were achieved by HPLC. In the investigated extracts, the most abundant phenolic was chlorogenic acid, followed by protocatechuic acid, while resveratrol, isoquercetin, quecetin and hyperoside were also present in significant quantities. Antioxidant potential was evaluated using two in vitro assays-FRAP and DPPH-being in the accordance with the cyclic voltammetry tests, performed as well. The results revealed that all the investigated extracts were rich in phenolic and essential mineral constituents, with significant antioxidant activity, depending on the polyphenolic and mineral contents, which was confirmed by principal component analysis.
本研究旨在建立 Montenegro 地区越橘植物化学成分与抗氧化活性的相关性。采用分光光度法测定果实和叶片提取物中的总酚、单宁、类黄酮、原花青素和花青素含量，采用电感耦合原子发射光谱仪测定金属含量。采用 HPLC 进行主要酚类物质的定性和定量分析。在所研究的提取物中，最丰富的酚类物质是绿原酸，其次是原儿茶酸，而白藜芦醇、异槲皮苷、桑色素和金丝桃苷的含量也相当可观。采用 FRAP 和 DPPH 两种体外测定法评估抗氧化能力，并进行了循环伏安法测试。结果表明，所有被研究的提取物都富含酚类物质和必需的矿物质成分，具有显著的抗氧化活性，这取决于多酚类物质和矿物质的含量，这一点通过主成分分析得到了证实。
