Tadić Vanja M, Nešić Ivana, Martinović Milica, Rój Edward, Brašanac-Vukanović Snežana, Maksimović Svetolik, Žugić Ana
Department for Pharmaceutical Research and Development, Institute for Medicinal Plant Research "Dr. Josif Pancic", Tadeusa Koscuska 1, 11000 Belgrade, Serbia.
Department of Pharmacy, Faculty of Medicine, University of Nis, Boulevard Dr. Zorana Djindjića 81, 18000 Nis, Serbia.
Antioxidants (Basel). 2021 Mar 16;10(3):465. doi: 10.3390/antiox10030465.
Bilberry represents a valuable source of antioxidant substances responsible for its application for the treatment of different conditions (such as inflammation, cardiovascular disease, cancer, diabetes, and different age-related diseases) associated with increased oxidative stress. As oxidative stress might cause skin impairments, we aim to evaluate a topical preparation containing bilberry leaves extract and bilberry seeds oil, obtained as a byproduct of the food industry. To obtain the extracts, the conventional maceration technique for leaves, and supercritical carbon dioxide extraction for seeds were employed. The chemical profile of both actives was achieved by HPLC and GC methods, revealing the presence of phenolic acids (chlorogenic being the most abundant), flavonoids (isoquercetin in the highest amount), and resveratrol in leaves extract, while in seeds oil the essential ω-3 and ω-6 fatty acids were determined in favorable ratio, almost being 1. Antioxidant potential of the wild bilberry extract and seed oil was evaluated using in vitro DPPH and FRAP assays. Finally, effects of the oil-in-water creams with mentioned wild bilberry isolates on the skin were investigated in an in vivo study conducted on healthy human volunteers, revealing the significant beneficial effects when topically applied.
越橘是抗氧化物质的宝贵来源，这使其可用于治疗与氧化应激增加相关的各种病症（如炎症、心血管疾病、癌症、糖尿病以及不同的与年龄相关的疾病）。由于氧化应激可能导致皮肤损伤，我们旨在评估一种含有越橘叶提取物和越橘籽油的外用制剂，该制剂是食品工业的副产品。为了获得提取物，叶采用常规浸渍技术，种子采用超临界二氧化碳萃取法。两种活性成分的化学特征通过高效液相色谱法（HPLC）和气相色谱法（GC）得以确定，结果显示叶提取物中存在酚酸（绿原酸含量最高）、黄酮类化合物（异槲皮苷含量最高）和白藜芦醇，而在籽油中，必需的ω-3和ω-6脂肪酸比例适宜，几乎为1。使用体外二苯基苦味酰基自由基（DPPH）和铁还原抗氧化能力（FRAP）测定法评估了野生越橘提取物和籽油的抗氧化潜力。最后，在对健康人类志愿者进行的体内研究中，考察了含有上述野生越橘分离物的水包油乳膏对皮肤的影响，结果表明局部应用时有显著的有益效果。
