Department of Plant Agriculture, Crop Science Building, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Theor Appl Genet. 2018 Oct;131(10):2157-2165. doi: 10.1007/s00122-018-3144-0. Epub 2018 Jul 26.
Key message Significant QTL for soyasaponin I, as the major component of the soyasaponin B, have been identified using an RIL soybean population, which could facilitate the development of functional food soybean cultivars. Soyasaponin B forms that are naturally found in soybean (Glycine max [L.] Merr.) seed, have been of interest to the food industry because of their functional food properties. The predominant form soyasaponin B is soyasaponin I. While some of the genes in the biosynthesis of soyasaponins have been characterized, the underlying genetics of soyasaponins as a quantitative trait is still poorly understood. The objective of this study was to identify quantitative trait loci (QTL) associated with the accumulation of soyasaponin I using a genetic mapping population. The population consisting of 186 F recombinant inbred lines derived from the cross of 'OAC Glencoe' and 'OAC Wallace' was grown in two Southern Ontario locations in 2015 and 2016. The concentration of soyasaponin I was determined using high-performance liquid chromatography. Putative QTL associated with the accumulation of soyasaponin I were identified through simple interval mapping and composite interval mapping on chromosomes 10 and 16, which explained up to 11% of the total phenotypic variation per QTL for the trait. A significant positive correlation was observed between soyasaponin I and seed protein concentration in the mapping population, which may be advantageous for the development of soybean lines with improved soyasaponin I profiles. QTL identified in this study may facilitate marker-assisted selection for the development of food-grade soybean lines with improved functional properties.
利用大豆重组自交系群体，鉴定出与大豆皂素 B 的主要成分大豆皂素 I 相关的重要数量性状位点（QTL），这将有助于功能性食品大豆品种的开发。天然存在于大豆（Glycine max [L.] Merr.）种子中的大豆皂素 B 形式因其具有功能性食品特性而受到食品工业的关注。大豆皂素 B 的主要形式是大豆皂素 I。虽然大豆皂素生物合成的一些基因已经得到了描述，但作为数量性状的大豆皂素的遗传基础仍知之甚少。本研究的目的是利用遗传图谱群体鉴定与大豆皂素 I 积累相关的数量性状位点（QTL）。该群体由 'OAC Glencoe' 和 'OAC Wallace' 杂交产生的 186 个 F 重组自交系组成，于 2015 年和 2016 年在安大略省南部的两个地点种植。使用高效液相色谱法测定大豆皂素 I 的浓度。通过简单区间作图和复合区间作图，在第 10 号和第 16 号染色体上鉴定出与大豆皂素 I 积累相关的假定 QTL，每个 QTL 最多可解释该性状总表型变异的 11%。在图谱群体中观察到大豆皂素 I 与种子蛋白质浓度之间存在显著正相关，这可能有利于开发具有改良大豆皂素 I 特征的大豆品系。本研究中鉴定的 QTL 可能有助于标记辅助选择，以开发具有改良功能特性的食品级大豆品系。
