电阻抗肌动描记术作为ActRIIB-mFc抑制肌生成抑制素的生物标志物：一项在野生型小鼠中的研究
Electrical impedance myography as a biomarker of myostatin inhibition with ActRIIB-mFc: a study in wild-type mice.
作者信息
Nagy Janice A, Kapur Kush, Taylor Rebecca S, Sanchez Benjamin, Rutkove Seward B
机构信息
Department of Neurology, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA.
Department of Neurology, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.
出版信息
Future Sci OA. 2018 Apr 16;4(6):FSO308. doi: 10.4155/fsoa-2018-0002. eCollection 2018 Jul.
AIM
We sought to determine the sensitivity of electrical impedance myography (EIM) to myofiber hypertrophy induced by treatment with various doses of ActRIIB-mFc, an inhibitor of myostatin signaling.
METHODS
Wild-type C57BL/6 J mice (n = 40, male) were treated with three different doses of ActRIIB-mFc (i.e., RAP-031) or vehicle twice weekly for 5 weeks. End point assessments included gastrocnemius EIM, force measurements, muscle mass and myofiber size quantification.
RESULTS
ActRIIB-mFc increased body mass, muscle mass and myofiber size across all doses. Alterations in EIM 50 kHz phase and center frequency () were also present, with trends in a dose-dependent fashion. Significant correlations between EIM parameters and myofiber/functional data were identified.
CONCLUSION
EIM outcomes can serve as effective biomarkers of myostatin signaling inhibition, demonstrating a dose sensitivity and correlation to standard assessments.
目的
我们试图确定电阻抗肌电图（EIM）对用不同剂量的肌生长抑制素信号抑制剂ActRIIB-mFc治疗诱导的肌纤维肥大的敏感性。
方法
将野生型C57BL/6 J小鼠（n = 40，雄性）每周两次用三种不同剂量的ActRIIB-mFc（即RAP-031）或赋形剂治疗，持续5周。终点评估包括腓肠肌EIM、力量测量、肌肉质量和肌纤维大小量化。
结果
所有剂量的ActRIIB-mFc均增加了体重、肌肉质量和肌纤维大小。EIM 50 kHz相位和中心频率（）也有改变，呈剂量依赖性趋势。确定了EIM参数与肌纤维/功能数据之间的显著相关性。
结论
EIM结果可作为肌生长抑制素信号抑制的有效生物标志物，显示出剂量敏感性并与标准评估相关。
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