Lipscomb M F, Ben-Sasson S Z, Uhr J W
J Immunol. 1977 May;118(5):1748-54.
Peritoneal exudate lymphocytes obtained from immune guinea pigs and cultured for 1 week on antigen-pulsed autologous macrophages were tested for their ability to bind to fresh antigen-pulsed autologous macrophages or to macrophages pulsed with an irrelevant antigen. Up to 30% of the lymphocytes bound to macrophages bearing the relevant antigen whereas only 2 to 5% remained nonspecifically bound to macrophages after vigorous washing. Specific binding was observed in cultures as early as 1 hr. Analysis of the kinetics of binding suggests that the observed nonspecific binding is not a step in specific binding. The possibility that weaker antigen-independent association between lymphocytes and macrophages precedes specific binding cannot be excluded. No evidence was obtained that serum antibody adsorbed to the macrophage or T cell plays a role in this cell interaction or that the T cell can bind antigen directly. We suggest that the observed specific binding represents the initial event in stimulation of T lymphocytes by antigen.
从免疫豚鼠获得的腹腔渗出淋巴细胞，在抗原脉冲的自体巨噬细胞上培养1周后，检测它们与新鲜抗原脉冲的自体巨噬细胞或与用无关抗原脉冲的巨噬细胞结合的能力。高达30%的淋巴细胞与携带相关抗原的巨噬细胞结合，而在剧烈洗涤后，只有2%至5%的淋巴细胞非特异性地与巨噬细胞结合。早在培养1小时后就观察到了特异性结合。结合动力学分析表明，观察到的非特异性结合不是特异性结合的一个步骤。不能排除淋巴细胞与巨噬细胞之间较弱的非抗原依赖性结合先于特异性结合的可能性。没有证据表明吸附在巨噬细胞或T细胞上的血清抗体在这种细胞相互作用中起作用，也没有证据表明T细胞可以直接结合抗原。我们认为，观察到的特异性结合代表了抗原刺激T淋巴细胞的初始事件。
