Schmitz H, Grass F
J Immunol Methods. 1986 Apr 3;88(1):115-20. doi: 10.1016/0022-1759(86)90059-1.
A 1-step method was developed to detect IgG antibody to human T-cell lymphotropic virus type III (HTLV-III). The supernatant of T-lymphocyte cultures infected with HTLV-III was incubated for several days with peroxidase-labelled anti-HTLV-III F(ab')2 antibody fragments. During this incubation period an enzyme-labelled immune complex (ELIC) was formed which could be stored at -20 degrees C. For the detection of anti-HTLV-III antibody only a single incubation step was required: ELIC and serum specimens were mixed in the wells of a microtitre plate coated with rheumatoid factor (RF). In the presence of HTLV-III specific antibody additional immune complexes form which are selectively bound to the immobilized RF. Compared with other routine procedures the test was able to detect antibody to HTLV-III with improved sensitivity and specificity.
开发了一种一步法来检测抗人类III型嗜T细胞病毒（HTLV - III）的IgG抗体。将感染HTLV - III的T淋巴细胞培养物的上清液与过氧化物酶标记的抗HTLV - III F(ab')2抗体片段孵育数天。在此孵育期间形成了一种酶标记免疫复合物（ELIC），它可以保存在-20℃。为了检测抗HTLV - III抗体，仅需要一个孵育步骤：将ELIC和血清标本在涂有类风湿因子（RF）的微量滴定板孔中混合。在存在HTLV - III特异性抗体的情况下，会形成额外的免疫复合物，它们会选择性地结合到固定化的RF上。与其他常规方法相比，该检测方法能够以更高的灵敏度和特异性检测抗HTLV - III抗体。
