National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
J Pharm Biomed Anal. 2018 Sep 10;159:548-566. doi: 10.1016/j.jpba.2018.07.030. Epub 2018 Jul 19.
In current work, targeted and non-targeted analysis of alkaloids and acetogenins from methanolic extracts of Asimina, Annona species and dietary supplements have been performed using UHPLC-QToF in positive ion mode. Thirty-five standard compounds (twelve alkaloids and twenty-three acetogenins) were used for the analysis. The fragment ions produced by collision induced dissociation (CID) revealed the characteristic cleavage and provided structural information. Aporphine alkaloids and acetogenins are the major groups found in Asimina and Annona species. An untargeted analysis based on high-resolution mass spectrometry was carried out to profile the alkaloids and acetogenins from Asimina species (As. triloba, As. parviflora). Magnoflorine, being a major alkaloid from twigs of As. triloba samples, was used as an example to discuss the fragmentation patterns. In (+)-ESI-MS, magnoflorine gave [M] ions at m/z 342.1705. The fragment ions at m/z 297.1127 [M-(CH)NH], 282.0886 [M-(CH)NH], 265.0865 [M-(CH)NH-CHOH], 237.0916 [M-(CH)NH-CHOH-CO], and 222.0681 [M-(CH)NH-CHOH-CO-CH] resulted from the [M] molecular ion. One dietary supplement claiming to contain paw paw (As. triloba) was also analyzed and showed a similar profile to twigs of As. triloba. A total of 131 compounds including standard compounds were identified from the different parts of As. triloba and As. parviflora samples. These compounds can be used to distinguish Asimina species. However, for definite identification of these unknown components, further investigation is required. This may provide a model for the rapid screening and structural characterization of bioactive constituents from plant extracts in a single analysis.
在目前的工作中，使用超高效液相色谱-飞行时间质谱（UHPLC-QToF）在正离子模式下对来自番荔枝科和番木瓜科植物甲醇提取物的生物碱和乙酰氧基酮进行了靶向和非靶向分析。使用了 35 种标准化合物（12 种生物碱和 23 种乙酰氧基酮）进行分析。碰撞诱导解离（CID）产生的碎片离子揭示了特征性的裂解，并提供了结构信息。阿朴啡生物碱和乙酰氧基酮是番荔枝科和番木瓜科植物中主要的化合物。基于高分辨率质谱的非靶向分析用于对番荔枝属（As. triloba、As. parviflora）植物中的生物碱和乙酰氧基酮进行分析。在 As. triloba 枝条中，作为主要生物碱的木兰花碱被用作一个例子来讨论其裂解模式。在 (+)-ESI-MS 中，木兰花碱给出 m/z 342.1705 的[M]离子。m/z 297.1127 [M-(CH)NH]、282.0886 [M-(CH)NH]、265.0865 [M-(CH)NH-CHOH]、237.0916 [M-(CH)NH-CHOH-CO]和 222.0681 [M-(CH)NH-CHOH-CO-CH]的碎片离子是由[M]分子离子产生的。一种声称含有木瓜（As. triloba）的膳食补充剂也进行了分析，结果与 As. triloba 枝条的图谱相似。从 As. triloba 和 As. parviflora 样品的不同部位共鉴定出 131 种化合物，包括标准化合物。这些化合物可用于区分番荔枝属植物。然而，对于这些未知成分的确切鉴定，需要进一步的研究。这可能为从植物提取物中快速筛选和结构鉴定生物活性成分提供了一个模型。
