Evaluation of antibacterial properties of hydroxyapatite/bioactive glass and fluorapatite/bioactive glass nanocomposite foams as a cellular scaffold of bone tissue.

AIMS AND OBJECTIVES

Infection is a serious problem for patients after implantation surgery, which is difficult to treat with antibiotic therapy. The present study was developed to evaluate and compare the antibacterial properties of hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/BG) nanocomposite foams as a cellular scaffold for use in bone defects by two macrodilution and disk diffusion methods.

MATERIALS AND METHODS

, and were cultured in brain heart infusion broth medium with nanocomposite powder for 5 days, and their bioactivity levels were evaluated by daily culturing on solid agar medium plates. To carry out the disk diffusion test, a disc form of nanocomposite foams was used on agar medium with 48 h incubation.

RESULTS

None of two nanocomposites even at their highest concentration (200 mg/mL) did not prevent the growth of two and microorganisms. However, HA/BG nanocomposite on the 3 day at a concentration of 200 mg/mL and on 4 and 5 day at a concentration of 100 mg/mL and FA/BG nanocomposite on the 4 day at a concentration of 100 mg/mL and on the 5 day at a concentration of 50 mg/mL could be able to kill microorganism. In the disc diffusion test, none of the nanocomposites could create a nongrowth zone. Both tested biomaterials showed increased antibacterial properties over time and concentration increase.

CONCLUSION

HA/BG and FA/BG nanocomposites, due to their biocompatibility and antimicrobial properties, are good choices for implantation instead of damaged bone tissue in tissue engineering.