Department of Operative Dentistry and Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran.
J Biomed Mater Res A. 2010 Jul;94(1):160-8. doi: 10.1002/jbm.a.32678.
The aim of this work was to evaluate the antibacterial effect of bioactive glass nanopowders. The 58S, 63S, and 72S compositions were prepared via the sol-gel technique. Characterization techniques such as X-ray diffraction, transmission electron microscopy (TEM), Zetasizer, and X-ray fluorescent were used. The antibacterial activity was studied using Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Staphylococcus aureus. Cytotoxicity of the samples was evaluated using mouse fibroblast L929 cell line. The chemical compositions of the prepared samples were as predicted, and the particle size of the samples with an amorphous structure mainly ranged over 20-90 nm. At broth concentrations below 50 mg/mL, they showed no antibacterial activity. The 58S showed the highest antibacterial activity with the minimum bactericidal concentrations of 50 and 100 mg/mL for E. coli plus S. aureus and for P. aeruginosa, respectively. The 63S exhibited bactericidal and bacteriostatic effects on E. coli and S. aureus at concentrations of 100 and 50 mg/mL, respectively, at an minimum bactericidal concentrations of 100 mg/mL. However, 72S bioactive glass nanopowder showed no antibacterial effect. They showed no cytotoxicity. It was concluded that bioactive glass nanopowders could be considered as good candidates for the treatment of oral bone defects and root canal disinfection. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
本工作旨在评估生物活性玻璃纳米粉末的抗菌效果。采用溶胶-凝胶技术制备了 58S、63S 和 72S 三种成分。使用了 X 射线衍射、透射电子显微镜（TEM）、Zetasizer 和 X 射线荧光等表征技术。使用大肠杆菌、铜绿假单胞菌、伤寒沙门氏菌和金黄色葡萄球菌研究了抗菌活性。使用小鼠成纤维细胞 L929 细胞系评估了样品的细胞毒性。所制备样品的化学成分与预期相符，具有无定形结构的样品的粒径主要在 20-90nm 之间。在低于 50mg/mL 的肉汤浓度下，它们没有表现出抗菌活性。58S 对大肠杆菌加金黄色葡萄球菌的最小杀菌浓度为 50 和 100mg/mL，对铜绿假单胞菌的最小杀菌浓度为 100mg/mL，表现出最高的抗菌活性。63S 在 100mg/mL 的浓度下对大肠杆菌和金黄色葡萄球菌分别表现出杀菌和抑菌作用，最小杀菌浓度为 100mg/mL。然而，72S 生物活性玻璃纳米粉末没有表现出抗菌作用。它们没有细胞毒性。结论是，生物活性玻璃纳米粉末可以被认为是治疗口腔骨缺损和根管消毒的良好候选物。（c）2010 年 Wiley 期刊，Inc. J 生物医学材料研究，2010 年。
