Yan Xin, Ebrahimi Ali, Mohammadi Reza, Tehrani Mona Mohajeri, Chaboki Pardis, Mustafa Mohammed, Alhumadi Almustafa, Sapaev I B, Hjazi Ahmed, Yousefi Khadije, Yousefi Mojdeh
The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Guangdong, China.
Foshan Hospital of Traditional Chinese Medicine, Foshan, China.
Sci Rep. 2025 Aug 5;15(1):28557. doi: 10.1038/s41598-025-12577-4.
The prolonged setting duration of mineral trioxide aggregate (MTA) constitutes one of its principal limitations. In contrast, Nano Fast Cement (NFC) emerges as an innovative nanocomposite characterized by a rapid setting time. To develop an outstanding dental filler, hydroxyapatite nanoparticles (NHA) were incorporated into NFC to evaluate their effects on setting time, biocompatibility, bioactivity, and antibacterial properties. Specimens containing 0, 10, and 20 weight% (W%) hydroxyapatite were subjected to assessment utilizing the MTT assay for cytotoxicity, the Gilmore needle for measuring setting time, scanning electron microscopy (SEM) and X-ray diffraction (XRD) for microstructural and phase analyses, as well as antibacterial evaluations against Enterococcus faecalis (PTCC 1394). Phase and microstructural investigations confirmed the formation of hydroxyapatite. The addition of 10 and 20 W% hydroxyapatite significantly improved the specimens' bioactivity while reducing toxicity by 20%. An increase in hydroxyapatite content corresponded with a greater enhancement in bioactivity and a further reduction in toxicity. These findings indicate that the addition of NHA did not detrimentally affect the physical properties of NFC; rather, it augmented the filler's bioactivity in vitro. Furthermore, the nanocomposite containing a higher concentration of NHA exhibited superior inhibition of E. faecalis compared to NFC in minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) (p-value < 0.001).
三氧化矿物凝聚体（MTA）较长的凝固时间是其主要局限性之一。相比之下，纳米速凝水泥（NFC）是一种具有快速凝固时间的新型纳米复合材料。为了开发一种出色的牙科填充材料，将羟基磷灰石纳米颗粒（NHA）掺入NFC中，以评估它们对凝固时间、生物相容性、生物活性和抗菌性能的影响。对含有0、10和20重量%（W%）羟基磷灰石的样本进行评估，使用MTT法检测细胞毒性，用吉尔摩针测量凝固时间，用扫描电子显微镜（SEM）和X射线衍射（XRD）进行微观结构和相分析，以及针对粪肠球菌（PTCC 1394）的抗菌评估。相和微观结构研究证实了羟基磷灰石的形成。添加10%和20% W%的羟基磷灰石显著提高了样本的生物活性，同时毒性降低了20%。羟基磷灰石含量的增加与生物活性的更大提高和毒性的进一步降低相对应。这些发现表明，添加NHA不会对NFC的物理性能产生不利影响；相反，它增强了填充材料的体外生物活性。此外，与NFC相比，含有较高浓度NHA的纳米复合材料在最低抑菌浓度（MICs）和最低杀菌浓度（MBCs）方面对粪肠球菌表现出更强的抑制作用（p值<0.001）。
