Xie Jinghang, Mu Ran, Fang Mingxi, Cheng Yunfeng, Senchyna Fiona, Moreno Angel, Banaei Niaz, Rao Jianghong
Departments of Radiology and Chemistry, Molecular Imaging Program at Stanford, Stanford University School of Medicine Stanford CA 94305 USA
Department of Pathology, Stanford University School of Medicine Stanford CA 94305 USA.
Chem Sci. 2021 May 28;12(26):9153-9161. doi: 10.1039/d1sc01471d. eCollection 2021 Jul 7.
The alarming increase of antimicrobial resistance urges rapid diagnosis and pathogen specific infection management. This work reports a rapid screening assay for pathogenic bacteria resistant to lactam antibiotics. We designed a fluorogenic N-cephalosporin caged 3,7-diesterphenoxazine probe CDA that requires sequential activations to become fluorescent resorufin. A series of studies with recombinant β-lactamases and clinically prevalent pathogens including , , and demonstrated that CDA possessed superior sensitivity in reporting the activity of β-lactamases including cephalosporinases and carbapenemases. After a simple filtration, lactam-resistant bacteria in urine samples could be detected at 10 colony-forming units per milliliter within 2 hours.
抗菌药物耐药性的惊人增长促使人们进行快速诊断和针对病原体的感染管理。这项工作报道了一种针对耐内酰胺类抗生素的病原菌的快速筛选检测方法。我们设计了一种荧光N-头孢菌素笼蔽的3,7-二酯吩恶嗪探针CDA，它需要顺序激活才能变成荧光试卤灵。一系列针对重组β-内酰胺酶以及包括 、 、 和 在内的临床常见病原体的研究表明，CDA在报告包括头孢菌素酶和碳青霉烯酶在内的β-内酰胺酶活性方面具有卓越的灵敏度。经过简单过滤后，尿液样本中每毫升含10个菌落形成单位的耐内酰胺细菌可在2小时内被检测到。
