Viral Hepatitis Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Infectious Disease Ambulatory, Clementino Fraga Filho Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
J Med Virol. 2018 Dec;90(12):1863-1867. doi: 10.1002/jmv.25275. Epub 2018 Sep 3.
This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination.
本研究旨在评估优化后的酶联免疫吸附试验（EIA）在人类免疫缺陷病毒（HIV）感染背景下检测和定量检测乙型肝炎表面抗体（抗-HBs）在干血斑（DBS）中的应用。从 56 名 HIV+和 99 名 HIV-患者中获得血清和 DBS 样本，并进行 EIA 检测抗-HBs，其中对 DBS 检测修改了样本体积和截止值。DBS 检测抗-HBs 的灵敏度分别为 HIV-和 HIV+患者的 79.8%和 76.8%。血清和 DBS 中抗-HBs 的一致性结果显示高平均 CD8 细胞计数、HIV 病毒载量和抗-HBs 的光密度（OD）值。无论 DBS 中是否检测到抗-HBs，血清中的抗-HBs 滴度均明显更高。在 HIV+和 HIV-患者中，分别可以检测到低至 17.4 和 27.3IU/mL 的 DBS 中的抗-HBs。总之，DBS 可用于检测和定量检测 HIV 感染个体中的抗-HBs，从而增加诊断和疫苗接种的机会。
