Robles L J, Breneman J W, Anderson E O, Nottoli V A, Kegler L L
Cell Tissue Res. 1986;244(1):115-20. doi: 10.1007/BF00218388.
Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons of Aplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or used whole for immunofluorescence studies. Ganglia were embedded in Lowicryl K4M for electron-microscope immunocytochemistry. In both the cerebral and abdominal ganglia, light-microscope immunocytochemical results showed reaction product deposited around the neuronal cell periphery corresponding in position to the lipochondria. In the abdominal ganglion, the giant cell R2, located in the right rostral quarter, and neurons in the right caudal quarter were consistently labeled with anti-opsin. Electron-microscopic studies demonstrated ferritin-labeling of the lipochondria in R2 and other immunoreactive neurons. Immunoblot analysis of R2 and cerebral neuron extracts was used to identify two prominent immunoreactive protein bands at 85 000 and 67 500 molecular weight.
针对鱿鱼视蛋白的多克隆抗体被用于免疫细胞化学和免疫印迹实验，以鉴定海兔光敏神经元中的一种视紫红质样蛋白。醛固定的腹神经节和脑神经节被包埋在石蜡中用于过氧化物酶抗过氧化物酶分析，或完整用于免疫荧光研究。神经节被包埋在Lowicryl K4M中用于电子显微镜免疫细胞化学。在脑神经节和腹神经节中，光学显微镜免疫细胞化学结果显示反应产物沉积在神经元细胞周边，其位置与脂粒体相对应。在腹神经节中，位于右吻侧四分之一处的巨细胞R2以及右尾侧四分之一处的神经元始终被抗视蛋白标记。电子显微镜研究表明R2和其他免疫反应性神经元中的脂粒体有铁蛋白标记。对R2和脑神经元提取物的免疫印迹分析用于鉴定分子量分别为85000和67500的两条突出的免疫反应性蛋白条带。
