Souza Ana Carolina Remondi, Fuchs Beth Burgwyn, Alves Viviane de Souza, Jayamani Elamparithi, Colombo Arnaldo Lopes, Mylonakis Eleftherios
Special Mycology Laboratory, Division of Infectious Diseases, Federal University of São Paulo-UNIFESP, 04039-032 São Paulo, SP, Brazil.
Division of Infectious Diseases, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI 02903, USA.
Genes (Basel). 2018 Aug 8;9(8):401. doi: 10.3390/genes9080401.
is a valuable tool as an infection model toward the study of species. In this work, we endeavored to develop a . - infection model by using the fungi as a food source. Three species of the C. parapsilosis complex ( (), and ) caused infection resulting in killing. All three strains that comprised the complex significantly diminished the nematode lifespan, indicating the virulence of the pathogens against the host. The infection process included invasion of the intestine and vulva which resulted in organ protrusion and hyphae formation. Importantly, hyphae formation at the vulva opening was not previously reported in . - infections. Fungal infected worms in the liquid assay were susceptible to fluconazole and caspofungin and could be found to mount an immune response mediated through increased expression of -, -, and /. Overall, the . -. infection model can be used to model . host-pathogen interactions.
作为研究[物种名称]感染模型的一种有价值工具。在这项工作中，我们致力于通过使用真菌作为食物来源来开发一种[具体感染模型名称]感染模型。近平滑念珠菌复合体的三个物种（[物种1名称]、[物种2名称]和[物种3名称]）引起感染导致[宿主名称]死亡。构成该复合体的所有三个菌株均显著缩短了线虫寿命，表明病原体对宿主的毒力。感染过程包括肠道和外阴的入侵，导致器官突出和菌丝形成。重要的是，以前在[具体感染情况]感染中未报道过外阴开口处的菌丝形成。液体试验中受真菌感染的蠕虫对氟康唑和卡泊芬净敏感，并且可以发现它们通过增加[基因名称1]、[基因名称2]和[基因名称3]的表达介导产生免疫反应。总体而言，[具体感染模型名称]感染模型可用于模拟[宿主名称]宿主 - 病原体相互作用。
