Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Dev Cell. 2018 Sep 10;46(5):564-580.e5. doi: 10.1016/j.devcel.2018.07.010. Epub 2018 Aug 9.
The olfactory epithelium (OE) is a neurosensory organ required for the sense of smell. Turbinates, bony projections from the nasal cavity wall, increase the surface area within the nasal cavity lined by the OE. Here, we use engineered fibroblast growth factor 20 (Fgf20) knockin alleles to identify a population of OE progenitor cells that expand horizontally during development to populate all lineages of the mature OE. We show that these Fgf20-positive epithelium-spanning progenitor (FEP) cells are responsive to Wnt/β-Catenin signaling. Wnt signaling suppresses FEP cell differentiation into OE basal progenitors and their progeny and positively regulates Fgf20 expression. We further show that FGF20 signals to the underlying mesenchyme to regulate the growth of turbinates. These studies thus identify a population of OE progenitor cells that function to scale OE surface area with the underlying turbinates.
嗅上皮（OE）是一种用于嗅觉的神经感觉器官。鼻甲是鼻腔壁上的骨性突起，增加了由 OE 衬里的鼻腔内的表面积。在这里，我们使用工程化的成纤维细胞生长因子 20（Fgf20）基因敲入等位基因来鉴定一个 OE 祖细胞群体，该群体在发育过程中水平扩张，以填充成熟 OE 的所有谱系。我们表明，这些 Fgf20 阳性的上皮跨越祖细胞（FEP）对 Wnt/β-Catenin 信号有反应。Wnt 信号抑制 FEP 细胞分化为 OE 基底祖细胞及其后代，并正向调节 Fgf20 表达。我们进一步表明，FGF20 信号传递到下面的间充质中，以调节鼻甲的生长。因此，这些研究确定了一个 OE 祖细胞群体，其功能是与下面的鼻甲一起扩大 OE 的表面积。
