School of Environmental Science , Nanjing Xiaozhuang College , Nanjing 211171 , PR China.
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) , Nanjing University of Posts and Telecommunications (NUPT) , Nanjing 210023 , PR China.
Inorg Chem. 2018 Sep 4;57(17):10625-10632. doi: 10.1021/acs.inorgchem.8b01272. Epub 2018 Aug 13.
Phosphorescence lifetime Zn imaging possesses the advantage over normal fluorescence imaging in offering the more accurate temporal-spatial Zn information. Herein, we report a new phosphorescent cyclometalated Ir(III) complex with a Zn-chelator bearing 1,10-phenanthrolin acting as ancillary ligand, Zin-IrDPA, which displays the specific Zn-induced enhancement of phosphorescence and phosphorescence lifetime, and the mitochondria-targeting ability. Moreover, its Zn-induced phosphorescence lifetime enhancement factor is not affected by medium lipophilicity, viscosity, polarity, and especially ambient oxygen. The reversible tracking of introduced exogenous labile Zn in MCF-7 and HeLa cells via phosphorescence imaging and phosphorescence lifetime imaging (PLIM) have been realized with Zin-IrDPA. Moreover, PLIM with Zin-IrDPA is able to track the SNOC-stimulated endogenous Zn release in mitochondria.
磷光寿命 Zn 成像在提供更准确的时空 Zn 信息方面优于常规荧光成像。在此，我们报道了一种新的磷光金属铱(III)配合物，其具有作为辅助配体的带有 1,10-菲咯啉的 Zn 螯合剂，即 Zin-IrDPA，它显示出特定的 Zn 诱导增强的磷光和磷光寿命以及线粒体靶向能力。此外，其 Zn 诱导的磷光寿命增强因子不受介质脂溶性、粘度、极性的影响，特别是环境氧的影响。通过磷光成像和磷光寿命成像（PLIM），使用 Zin-IrDPA 实现了对 MCF-7 和 HeLa 细胞中引入的外部分解 Zn 的可逆追踪。此外，使用 Zin-IrDPA 的 PLIM 能够追踪 SNOC 刺激的线粒体中内源性 Zn 的释放。
