a Department of Anatomy, Division of Basic Medicine , Tokai University School of Medicine , Kanagawa , Japan.
b Graduate School of Human Environmental Studies, Course of Human Environmental Studies , Tokai University , Kanagawa , Japan.
Int J Environ Health Res. 2018 Dec;28(6):683-696. doi: 10.1080/09603123.2018.1504897. Epub 2018 Aug 13.
Neonicotinoids, such as acetamiprid (ACE), a pesticide used worldwide, are believed to be safe for human use. These molecules are structurally similar to nicotine, act as nicotinic acetylcholine receptor (nAChR) agonists, and were shown to be associated with neuromuscular and reproductive disorders, but these experiments were primarily performed in mature animals. In this study, the effects of ACE on the testes of immature mice were examined. The exposure of 3-week-old mice to ACE-containing water for 180 days led to a decrease in body weight and mildly affected spermatogenesis. Additionally, the expression of testosterone-metabolism genes, nAChR subunit genes, and proliferation-associated genes decreased in the testes of ACE-treated mice. Our results show that immature rodents may be less sensitive to ACE than mature ones, that mice may be more likely to accumulate ACE than rats, and that the development of disorders may be affected by the accumulation of ACE in the testes.
新烟碱类，如乙酰甲胺磷（ACE），一种在世界范围内使用的农药，被认为对人类使用是安全的。这些分子结构上与尼古丁相似，作为烟碱型乙酰胆碱受体（nAChR）激动剂，被证明与神经肌肉和生殖系统紊乱有关，但这些实验主要在成熟动物中进行。在这项研究中，研究了 ACE 对未成年小鼠睾丸的影响。将 3 周龄的小鼠暴露在含有 ACE 的水中 180 天，导致体重下降，精子发生受到轻微影响。此外，ACE 处理小鼠睾丸中睾丸酮代谢基因、nAChR 亚基基因和增殖相关基因的表达降低。我们的结果表明，未成熟的啮齿动物可能比成熟的啮齿动物对 ACE 的敏感性更低，而且与大鼠相比，小鼠可能更容易积累 ACE，ACE 在睾丸中的积累可能会影响疾病的发展。
