Chi Yujie, Liu Changli, Liu Wei, Tian Xufang, Hu Juan, Wang Bo, Liu Di, Liu Yifei
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
Hubei Institute for Drug Control, Wuhan, China.
Front Plant Sci. 2024 Mar 19;15:1341996. doi: 10.3389/fpls.2024.1341996. eCollection 2024.
The rhizomes of plants have been used in traditional Chinese medicine over 2000 years. Due to increasing market demand, the overexploitation of wild populations, habitat degradation and indiscriminate artificial cultivation of species have severely damaged the native germplasms of species in China.
Genome-wide simple-sequence repeat (SSR) markers were developed using the genomic data of . Population genetic diversity and structure of 345 accessions collected from 32 different populations were performed based on these SSRs. The distribution of suitable areas for three taxa in China was predicted and the effects of environmental variables on genetic diversity in relation to different population distributions were further analyzed.
22 primer pairs were selected as clear, stable, and polymorphic SSR markers. These had an average of 16.41 alleles and an average polymorphism information content (PIC) value of 0.664. In the neighbor-joining (N-J) clustering analysis, the 345 individuals clustered into three groups, with , var. and being clearly separated. All accessions were further divided into four subgroups in the population structure analysis. The predicted distributions of suitable areas and the environmental variables shaping these distributions varied considerably among the three species.
Overall, the amount of solar radiation, precipitation and altitude were the most important environmental variables influencing the distribution and genetic variation of three species. The findings will provide key information to guide the conservation of genetic resources and construction of a core reserve for species.
植物的根茎在中国传统医学中已被使用了2000多年。由于市场需求的增加、野生种群的过度开发、栖息地退化以及物种的无序人工种植，已严重破坏了中国物种的原生种质。
利用……的基因组数据开发全基因组简单序列重复（SSR）标记。基于这些SSR对从32个不同种群收集的345份材料进行群体遗传多样性和结构分析。预测了中国三种分类群适宜区域的分布，并进一步分析了环境变量对不同种群分布相关遗传多样性的影响。
选择了22对引物作为清晰、稳定且具有多态性的SSR标记。这些标记平均有16.41个等位基因，平均多态性信息含量（PIC）值为0.664。在邻接法（N-J）聚类分析中，345个个体聚为三组，……、……变种和……明显分开。在群体结构分析中，所有材料进一步分为四个亚组。三种物种适宜区域的预测分布以及塑造这些分布的环境变量差异很大。
总体而言，太阳辐射量、降水量和海拔是影响三种物种分布和遗传变异的最重要环境变量。这些发现将为指导物种遗传资源保护和核心保护区建设提供关键信息。
