Białas Adam J, Liberski Paweł P, Zielińska Anna, Kumor-Kisielewska Anna, Szewczyk Karolina, Miłkowska-Dymanowska Joanna, Sitarek Przemysław, Piotrowski Wojciech J, Górski Paweł
Department of Pneumology and Allergy, Medical University of Łódź, Łódź, Poland,
Department of Molecular Pathology and Neuropathology, Medical University of Łódź, Łódź, Poland.
Int J Chron Obstruct Pulmon Dis. 2018 Jul 30;13:2313-2318. doi: 10.2147/COPD.S163249. eCollection 2018.
Exacerbations of chronic obstructive pulmonary disease (ECOPD) are important events in the course of the disease, negatively influencing health status and disease progression. Therefore, there is a strong need for deeper understanding of the pathology of ECOPD to elaborate new therapeutic approaches and ameliorate prognoses. Contributions of mitochondria to pathobiology of COPD are still under investigation, although growing evidence suggests their important role in this disease. The aim of our study was to assess the morphometric parameters of mitochondria in lymphocytes of patients with ECOPD.
Lymphocytes were isolated from the peripheral blood of patients with COPD. Transmission electron microscopy was used to assess absolute number of mitochondria per cell, mitochondrial content, and morphometric parameters of individual mitochondria. We also counted indexes for elongation and interconnectivity.
Eighteen patients (9 with ECOPD and 9 in the stable period of the disease) were analyzed. We observed significantly lower length of mitochondrion (=0.03) and significant decrease both in elongation (=0.03) and interconnectivity indexes (=0.04) in ECOPD patients.
The morphometric parameters of mitochondria in lymphocytes derived from patients during the early period of ECOPD requiring hospitalization are altered in comparison to patients in the stable period of the disease. This suggests their contribution to pathobiology of ECOPD. These preliminary outcomes should be further validated in larger size samples.
慢性阻塞性肺疾病急性加重（ECOPD）是该疾病病程中的重要事件，对健康状况和疾病进展产生负面影响。因此，迫切需要更深入地了解ECOPD的病理机制，以制定新的治疗方法并改善预后。尽管越来越多的证据表明线粒体在慢性阻塞性肺疾病（COPD）中起重要作用，但线粒体对COPD病理生物学的贡献仍在研究中。本研究的目的是评估ECOPD患者淋巴细胞中线粒体的形态计量学参数。
从慢性阻塞性肺疾病患者的外周血中分离淋巴细胞。使用透射电子显微镜评估每个细胞中线粒体的绝对数量、线粒体含量以及单个线粒体的形态计量学参数。我们还计算了伸长率和相互连接性指数。
对18名患者（9名ECOPD患者和9名疾病稳定期患者）进行了分析。我们观察到ECOPD患者的线粒体长度显著缩短（=0.03），伸长率（=0.03）和相互连接性指数（=0.04）均显著降低。
与疾病稳定期患者相比，因ECOPD早期需要住院治疗的患者淋巴细胞中线粒体的形态计量学参数发生了改变。这表明它们对ECOPD病理生物学有贡献。这些初步结果应在更大规模的样本中进一步验证。
