Zuffa A, Zajac J, Zuffa T
Vet Med (Praha). 1986 Apr;31(4):209-18.
Trials were conducted on rabbits and cattle to compare the immunizing effectiveness of the subunit vaccine against infectious bovine rhinotracheitis (IBR), representing antigens separated by the solubilization of the IBR virus-infected cells by means of Triton X-100 with oil adjuvant, with the inactivated oil IBR vaccine. The rabbits inoculated and re-vaccinated with both vaccines in an interval of three weeks produced neutralizing antibodies in medium titres, the values of these antibodies were balanced in both groups. Cattle immunized with the subunit vaccine reacted to the inoculation and re-vaccination by producing serum antibodies of higher titres, as compared with the cattle inoculated with the virus vaccine; secretory antibodies were detected only after re-vaccination and had balanced values in both test groups. After intranasal infection with the virulent virus performed after 14 days from re-vaccination, the calves inoculated with the subunit and virus vaccines were protected against clinical disease whereas the non-inoculated control calves fell ill with symptoms characteristic of IBR. The immunized animals of both experimental groups had a smaller amount of virus p.i. in nasal secretions and for a shorter time than the control non-inoculated calves. The intensity of multiplication and persistence of infectious virus excretion were the same in both experimental groups.
在兔子和牛身上进行了试验，以比较亚单位疫苗与灭活油佐剂传染性牛鼻气管炎（IBR）疫苗对IBR的免疫效果。亚单位疫苗的抗原是通过用Triton X-100溶解IBR病毒感染的细胞而分离出来的，并与油佐剂混合。每隔三周用两种疫苗接种和再次接种的兔子产生了中等滴度的中和抗体，两组抗体值相当。与接种病毒疫苗的牛相比，用亚单位疫苗免疫的牛对接种和再次接种的反应是产生更高滴度的血清抗体；仅在再次接种后检测到分泌性抗体，且两个试验组的分泌性抗体值相当。在再次接种后14天进行鼻内强毒病毒感染后，接种亚单位疫苗和病毒疫苗的小牛对临床疾病具有抵抗力，而未接种的对照小牛则出现IBR特征性症状。与未接种的对照小牛相比，两个实验组的免疫动物鼻分泌物中的病毒感染量更少，持续时间更短。两个实验组中传染性病毒排泄的增殖强度和持续时间相同。
