González E, Molina R, Jiménez M
Laboratorio de Entomología Médica, Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain.
Laboratorio de Entomología Médica, Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain.
Acta Trop. 2018 Nov;187:201-206. doi: 10.1016/j.actatropica.2018.08.011. Epub 2018 Aug 11.
Phlebotomine sand flies are known vectors of several pathogens. In Spain, Phlebotomus perniciosus and Phlebotomus ariasi are the proven vectors of Leishmania infantum. Since 2010 a human leishmaniasis outbreak has been notified in Madrid region, central Spain. Studies have shown that P. perniciosus is the only vector confirmed in the focus area and that rabbits and hares are the wild reservoirs incriminated in the cycle of the parasite in the outbreak. Trypanosoma nabiasi is a trypanosomatid found in wild rabbits and its presence has been reported in wild rabbits from southern Spain. Moreover, co-infection with L. infantum was found in some of these animals. However, in Madrid region, there is no information about the transmission of this trypanosome in rabbits. Hence, in this study we investigate if T. nabiasi could be circulating in the aforementioned leishmaniasis focus. Wild P. perniciosus female sand flies were captured in the affected area and analyzed using molecular methods. T. nabiasi DNA was detected in 20 out of 155 female sand flies fed on rabbits by amplification and subsequent sequencing of ITS1 and SSU rRNA fragments. Therefore, we describe for the first time the presence of T. nabiasi and its co-infection with L. infantum in P. perniciosus female sand flies. More investigation is needed in order to elucidate the role of P. perniciosus in the transmission of T. nabiasi among rabbits and its potential consequences.
白蛉是几种病原体的已知传播媒介。在西班牙，长须白蛉和阿氏白蛉是婴儿利什曼原虫的已证实传播媒介。自2010年以来，西班牙中部马德里地区已通报了一起人类利什曼病疫情。研究表明，长须白蛉是疫源地唯一确认的传播媒介，兔子和野兔是此次疫情中寄生虫传播周期中被怀疑的野生动物宿主。纳氏锥虫是在野兔中发现的一种锥虫，西班牙南部的野兔中已报告有其存在。此外，在其中一些动物中发现了与婴儿利什曼原虫的共同感染。然而，在马德里地区，没有关于这种锥虫在兔子中传播的信息。因此，在本研究中，我们调查了纳氏锥虫是否可能在上述利什曼病疫源地传播。在受影响地区捕获野生长须白蛉雌蛉，并使用分子方法进行分析。通过对ITS1和SSU rRNA片段进行扩增和后续测序，在155只以兔子为食的雌蛉中的20只中检测到了纳氏锥虫DNA。因此，我们首次描述了长须白蛉雌蛉中纳氏锥虫的存在及其与婴儿利什曼原虫的共同感染。为了阐明长须白蛉在纳氏锥虫在兔子之间传播中的作用及其潜在后果，还需要进行更多的研究。
